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西宝生物
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文献和实验Characterization and Validation of Cre‐Driver Mouse Lines
animals Ppm1a‐CreERT2 /RXRαΔAF2(LNL) and Vil1‐CreERT2 /RXRαΔAF2(LNL) mice injected with vehicle (top) or with tamoxifen (bottom). For tamoxifen injections, tamoxifen (Sigma, cat. no. T56648) was prepared at 10 mg/ml in sunflower seed oil (Sigma, cat. no. S
until DEPC is well distributed and then autoclave. Prepare enough of this to use in all other solutions. 2. Labeling buffer (0.1 M NaHCO3, pH 9.0): To make 100 ml, weigh 0.84 g NaHCO3 and complete to 100 ml with DDW. Adjust pH to 9.0 by adding 10N NaOH
buffer (0.1 M Na2CO3 sodium carbonate, pH 9.0): To make 100 ml, weigh 1.06 g Na2CO3 and complete to 100 ml with DDW. Adjust pH to 9.0 by adding 10N NaOH and store at 4°C. 3. 2M TEAB stock: To make 500 ml, take 138.3 ml (101 g) TEA
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