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文献和实验High-Throughput Expression in Microplate Format in Pichia pastoris
-well format. Lysis of the cells occurs via chemical lysis without mechanical disruption. Using the optimized feeding and induction protocol, we are now able to screen for and identify expression clones that produce heterologous protein with a yield of 2 mg
High-Throughput Expression in Microplate Format in Saccharomyces cerevisiae
We have developed a high-throughput technology that allows parallel expression, purification, and analysis of large numbers of cloned cDNAs in the yeast Saccharomyces cerevisiae . The technology is based on a vector for intracellular protein
in 96-well format" and "High-quality DNA for reliable multiplex PCR analysis" and table "High-quality RNA from tissues"), and without the need for additional RNase or DNase digestion (see figure "RNA with no genomic DNA contamination").
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