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1-THIOGLYCEROL "CONTAINS 10% WATER" ([OD]2, S-D, 98%)
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文献和实验Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
Replication timing by comparative hybridization
spread after Pronase treatment aren't contaminated--but I heard from someone who'd used Sigma's Pronase and had massive growth of Streptomyces.
contains 1.98ml of diluent mixture: Ammonium oxalate 11.45gm, Sorensen`s phosphate buffer 1.0gm , Thimerosal 0.1gm, Purified water qs to 1L ADDITIONAL INFO For automatic counts you can use
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