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文献和实验Sphingomyelin Quantitation Post-choline Labeling of HL60 Cells
. 17) Add 50 µl of SMase solution to each reaction tube and allow reaction to proceed for 30 minutes. --> Remember to have a control tube containing no enzyme to determine background. 18) Add 1.5 ml Chloroform/Methanol (2:1) to each tube to stop
Sphingomyelin Quantitation Post-choline Labeling of HL6
Sphingomyelin Quantitation Post-choline Labeling of HL60 Cells Contributor: Suprya Jayadev Date: Jun. 16, 1992 Reagents TMT buffer: 0.19 M Tris-HCl (pH 7.4-7.6) 12 mM MgCl2 0.2 % Triton
Sphingomyelin Quantitation Post-choline Labeling of HL60 Cells
Sphingomyelin Quantitation Post-choline Labeling of HL60 Cells Contributor: Suprya Jayadev Date: Jun. 16, 1992 Reagents TMT buffer: 0.19 M Tris-HCl (pH 7.4-7.6) 12 mM MgCl2 0.2 % Triton X-100 SMase solution
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