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文献和实验Using N,N,N,N-tetramethyl-p-phenylenediamine (TMPD) to Assay Cyclooxygenase Activity In Vitro
-phenylenediamine (TMPD) in high throughput microplate assays of COX activity could become the approach of choice in the screening of potential therapeutics that inhibit COX activity in vivo. Considering that TMPD is also a potential substrate
chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1, LO/IMEX) 8. reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. B. SOLUTIONS 1. PBS-PF (paraformaldehyde), 2% 2. PBS-tween 20, 0.05% 3. glycine (x
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
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