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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Sample Buffer Solution with
- 库存:
大量
- 供应商:
Wako(和光纯药工业株式会社)
- 规格:
25ml
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文献和实验Reducing Sample Complexity in Proteomicsby Chromatofocusing with Simple Buffer Mixtures
Chromatofocusing has many potential applications in the field of proteomics, such as for the isolation and removal of major sample components to facilitate the analysis of low-abundance components, and for sample prefractionation
Sample preparation (analytical gels)
, rinsed with cooled phosphate buffer saline, pH 7.2 to further remove cell debris and blood and then immersed into an iced PBS solution containing 3 mM EDTA, 50 μg/ml leupeptine, 0.2 mM PMSF and 0.8 mM benzamidine. Crypts were scraped away from the basal
Sample preparation (preparative gels)
Human samples CEC (Colon epithelial cell): Within a maximum of 30 min, a large right or left colon tissue sample (5 x 7 cm) was prepared on ice in the operating room, rinsed with cooled phosphate buffer saline, pH 7.2 to further remove
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