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文献和实验. In this chapter, we describe the development of an alternative, structurally simple, synthetic biodegradable electrospun scaffold based on poly(lactide-co -glycolide) (PLGA: materials used in dissolvable sutures) to replace AM.
)-cellulose consists of a polymer of 10–20 T-residues, covalently linked to a cellulose matrix, which will hybridize to and bind poly(A)+ containing RNA providing the tail is at least 15–20 bases long. Ribosomal and transfer RNA do not bind efficiently
. Procedure Add 1/10 volume 3 M NaAc, pH 5.2, and 2.5 volumes 100% ethanol. Mix and incubate at -20C for at least 1 hour. Centrifuge at > 12,000 x g in a microfuge for 20 minutes at 4C.
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