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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商:
上海西宝
- 英文名:
hMeDIP Assay
- 规格:
10 rxns
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文献和实验Hydroxymethylated DNA Immunoprecipitation (hmeDIP)
to assay for canonical 5-methylcytosine (5mC) cannot distinguish between 5hmC and 5mC. The development of antibodies specific to 5hmC has allowed for specific enrichment of DNA fragments containing 5hmC. Hydroxymethylated DNA immunoprecipitation (hmeDIP
*** Make sure the agar equilibrates to 55 C to 60 C before starting the assay; otherwise it will kill the cells. 3. Distribute several paper disks (Difco, #1599-33-6) into a sterile petri dish, and spot either 5 or 15 of synthetic alpha-factor (1 - 5 mg
Clonality - X Chromosome Inactivation Assay
. Clonal cell populations will show "loss" of the non-methylated allele after restriction digest. The assay can be performed on DNA recovered from microdissected samples. Both frozen tissue and fixed-embed allowfullscreen='true'ded archival tissue
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