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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
A c-Kit (2B8) FITC-IgG2b, 01904D FITC-IgG2b Isotype control, 11184C Fc RII block (clone 2.4G2), 01241D Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated
Retroviral Gene Expression Control in Primary Organoid Cultures
of transgenic mouse lines. Establishing such animal models is laborious, expensive, and time?consuming. Ever?expanding endodermal organoids, grown in an in vitro 3?D epithelial culture system, faithfully recapitulate the in vivo counterpart and represent
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