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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
10年
- 英文名：
北诺
- 库存：
1000
- 供应商：
北诺
- 规格：
20个/包
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文献和实验).Transfer serum/white blood cells to 50 ml tube, underlay 12 ml RT Histopaque 1077.Spin 2500 rpm/30min at RT. To take mononuclear cells:Take off serum above band. (See attached figure, in preparation)Take mononuclear band + some Ficoll into two 15ml tubes
Cleaning worms by floating on sucrose
tube Wash off with M9, S-basal, or water Be sure to grow worms on agarose plates when making DNA to be cut Wash worms in water. This is optional, it makes them more buoyant For a 15ml tube, spin in clinical centrifuge
vigorous vortexing. 2. Divide the diluted nuclei into 2x15ml portions. Overlay each portion onto 15ml of S2 solution in a 50 ml Falcon tube. Make sure the interface of the two layers is sharp (Figure 1).
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