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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4度,-20
- 保质期:
2年
- 英文名:
thermo
- 库存:
1000
- 供应商:
北诺生命科学
- 规格:
1 ml
货号 产品名称 规格 2013 目录价(¥)
K0331 Luminaris Color Probe High ROX qPCR Master Mix 250 x 20 µL rxns 1,355
K0332 Luminaris Color Probe High ROX qPCR Master Mix 500 x 20 µL rxns 2,510
K0333 Luminaris Color Probe High ROX qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0334 Luminaris Color Probe High ROX qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0341 Luminaris Color Probe Low ROX qPCR Master Mix 250 x 20 µL rxns 1,355
K0342 Luminaris Color Probe Low ROX qPCR Master Mix 500 x 20 µL rxns 2,510
K0343 Luminaris Color Probe Low ROX qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0344 Luminaris Color Probe Low ROX qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0351 Luminaris Color Probe qPCR Master Mix 250 x 20 µL rxns 1,355
K0352 Luminaris Color Probe qPCR Master Mix 500 x 20 µL rxns 2,510
K0353 Luminaris Color Probe qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0354 Luminaris Color Probe qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0361 Luminaris Color HiGreen High ROX qPCR Master Mix 250 x 20 µL rxns 1,355
K0362 Luminaris Color HiGreen High ROX qPCR Master Mix 500 x 20 µL rxns 2,510
K0363 Luminaris Color HiGreen High ROX qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0364 Luminaris Color HiGreen High ROX qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0371 Luminaris Color HiGreen Low ROX qPCR Master Mix 250 x 20 µL rxns 1,355
K0372 Luminaris Color HiGreen Low ROX qPCR Master Mix 500 x 20 µL rxns 2,510
K0373 Luminaris Color HiGreen Low ROX qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0374 Luminaris Color HiGreen Low ROX qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0381 Luminaris Color HiGreen Fluorescein qPCR Master Mix 250 x 20 µL rxns 1,355
K0382 Luminaris Color HiGreen Fluorescein qPCR Master Mix 500 x 20 µL rxns 2,510
K0383 Luminaris Color HiGreen Fluorescein qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0384 Luminaris Color HiGreen Fluorescein qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0391 Luminaris Color HiGreen qPCR Master Mix 250 x 20 µL rxns 1,355
K0392 Luminaris Color HiGreen qPCR Master Mix 500 x 20 µL rxns 2,510
K0393 Luminaris Color HiGreen qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0394 Luminaris Color HiGreen qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0441 TranscriptAid™ T7 High Yield Transcription Kit 50 react 2,325
K0512 Genomic DNA Purification Kit 100 preps 595
K0513 Silica Bead DNA Gel Extraction Kit 100 preps 541
K0621 DecaLabel™ DNA Labeling Kit 10 react 541
K0622 DecaLabel™ DNA Labeling Kit 30 react 1,352
K0651 Biotin DecaLabel™ DNA Labeling Kit 10 react 919
K0652 Biotin DecaLabel™ DNA Labeling Kit 30 react 2,271
K0661 Biotin Chromogenic Detection Kit 10 react 865
K0662 Biotin Chromogenic Detection Kit 30 react 1,947
K1071 DreamTaq™ PCR Master Mix (2X) 200 react 541
K1072 DreamTaq™ PCR Master Mix (2X) 1000 react 2,163
K1081 DreamTaq™ Green PCR Master Mix (2X) 200 react 541
K1082 DreamTaq™ Green PCR Master Mix (2X) 1000 react 2,163
K1091 Fast Tissue-to-PCR Kit 200 react 2,271
K1213 InsTAclone™ PCR Cloning Kit 10 react 649
K1214 InsTAclone™ PCR Cloning Kit 30 react 1,406
K1231 CloneJET™ PCR Cloning Kit 20 react 1,146
K1232 CloneJET™ PCR Cloning Kit 40 react 2,163
K1422 Rapid DNA Ligation Kit 50 react 1,179
K1423 Rapid DNA Ligation Kit 150 react 2,358
K1611 First Strand cDNA Synthesis Kit 20 react 595
K1612 First Strand cDNA Synthesis Kit 100 react 2,379
K1621 RevertAid™ First Strand cDNA Synthesis Kit 20 react 433
K1622 RevertAid™ First Strand cDNA Synthesis Kit 100 react 1,730
K1631 RevertAid™ H Minus First Strand cDNA Synthesis Kit 20 react 757
K1632 RevertAid™ H Minus First Strand cDNA Synthesis Kit 100 react 3,028
K1641 Maxima® First Strand cDNA Synthesis Kit 50 react 2,704
K1642 Maxima® First Strand cDNA Synthesis Kit 200 react 8,111
K1651 RevertAid™ Premium First Strand cDNA Synthesis Kit 20 react 1,049
K1652 RevertAid™ Premium First Strand cDNA Synthesis Kit 100 react 4,196
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文献和实验trick 现在大部分的文章做到PKC的活化都是通过western检测磷酸化,这个我也做过,但是不太明显,所以想从pkc转位这个角度做它的活化研究。。 但是看了一些文献和综述,conventional pkc和novel pkc这样检测他们的活化都是没有问题的,但是有的综述上说atypical pkc的活化是不发生转位的,因为它相对与前2者缺乏一个佛波酯激活域,但是我也看到一些文献做atypical pkc的活化也是通过做他的转位的,就很困
licanming 请问有无人用过assaydesigns公司的PKC Kinase Activity Assay Kit(cat NO:EKS-420A)?可否说一下应用的经验?注意事项等?还有,该试剂盒的说明书有几处地方不是很懂,请大家帮忙看一下: 1、To avoid cross contamination, change disposable pipette tips between the addition of each standard
三七家园 呵呵,希望大侠们,大牛们,前辈们不吝赐教。。。。:P licanming 可以讲具体一点吗? 三七家园 谢谢楼上的关心,我现在要检测pkc三个亚基δ,ε,ζ在用0.2uM的PMA(pkc的激动剂)后如何被激活?(是被磷酸化呢?还是蛋白量表达增加?或者别的什么?)怎样检测这种激活?(如果是前两个,我想用western blot因为我现在只会这个技术) ffyy
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