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- CST
- USA
- 9717S
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20
- 保质期：
2-10年
- 英文名：
Cell Signaling Technology
- 库存：
1000
- 供应商：
CST
- CAS号：
Monoclonal Antibody
- 规格：
100 ul
美国 Cell Signaling Technology（CST）是最知名的和老牌的信号转导公司之一，专注于信号转导产品的提供和研究。产品具有种类多、质量好、价格低、引用文献多等优点，是您进行信号转导研究不可多得的帮手！
提供特色的信号传导用磷酸化检测抗体、ELISA检测试剂盒、活性的重组激酶等药筛产品。涉及的细胞信号通路有： 1 染色质调控与乙酰化信号通路 2 MAP激酶信号网络 3 SAPK/JNK信号通路 4 细胞凋亡与Caspase信号网络 5 AKT信号网络 6 转录与翻译调控 7 蛋白激酶C PKC 信号网络 8 周期调控与DNA损伤信号通路 9 细胞骨架调控 10 白细胞信号传导，等等。
主要产品：
抗体类：提供超过1300种磷酸化和非磷酸化抗体，具有最多的磷酸化抗体，同时提供sample kit和phosphoPlus Ab kit
ELISA试剂盒：PathScan Sandwich ELISA Kit系列产品是检测蛋白磷酸化水平的ELISA试剂盒，灵敏度高，操作简单
WB用蛋白Marker：CST的Biotinylated Protein Ladder Detection Pack产品包括有10个条带、大小在10-200Kda之间的生物素标记蛋白标准和HRP藕联的抗生物素二抗（anti-biotin antibody, HRP-linked），有大小两种包装可选
kinase assay kit：试剂盒组成合理全面，非同位素操作，应用起来简单方便。
buffer类：共有6个buffer类，是进行普通WB或磷酸化WB检测的必须辅助试剂，用于标本蛋白的制备。
激酶类：多种形式的蛋白激酶，可以用于药物筛选
药物筛选试剂盒：高通量、针对激酶的药物筛选
The genomic era has yielded a wealth of new genes for which function and inter-relationships remain unknown. At the same time there is an increasing appreciation of the limits of what can be learned from gene expression profiling, and of the need to examine protein expression and activity. Activation-state-specific antibodies have proven to be a convenient and reliable set of tools in the new post-genomic "research toolbox". Cell Signaling Technology, Inc. （CST） is committed to developing innovative new research tools such as these to help define the mechanisms underlying cell function and disease, thereby broadly accelerating progress in biomedical research and medicine.
CST strives to make novel antibodies of the highest possible quality by producing them in-house and rigorously characterizing them with respect to specificity and sensitivity. Production begins with extensive purification （typically by both protein A and peptide affinity chromatography） followed by testing by our staff scientists on a wide range of applications, including ELISA, western blotting, immunoprecipitation, immunocytochemistry, immunohistochemistry and flow cytometry. Technical support is provided by the very scientists who make a given antibody and know it best. CST scientists are also responsible for creating most of the cell signaling reference material, including pathways, kinase trees and kinase disease associations that we offer our customers in the last chapter of this catalog and on our website.
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文献和实验Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
to chromatin, by its histone tail interaction or by other interactions, then you would not expect to see loss of binding upon peptide competition. A good positive control for the assay is to use H3K9 dimethylated peptide to compete HP1 from the pericentric
2B12) XP® Rabbit mAb (ChIP Formulated) #4620。该抗体可检测与基因组中所有 DNA 序列结合的所有组蛋白 H3 类型（H3、H3.3、CENP-A）。因此，不管检测的位点活性状态如何，该抗体都能为 ChIP 实验提供通用的阳性对照。阴性对照阴性对照抗体（如正常兔 IgG）不能识别特异抗原表位，因此可用于检测非特异性结合。例如，如果阴性对照样品中的产物量等于特异靶标样品中产物量，那么可得出结论，特异靶标抗体出现非特异结合或本底水平的信号。这个结果结合阳性组蛋白 H3
INTRODUCTION In cells and tissues, the histone proteins that constitute the nucleosomes can present multiple post-translational modifications, such as lysine acetylation, lysine and arginine methylation, serine phosphorylation
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