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OXOTREMORINE SESQUIFUMARATE
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文献和实验Immunofluorescence on ultrathin resin sections
Immunofluorescence on ultrathin resin sections This protocol is taken from the 1999 FEBS course manual and was contributed by Heinz Schwarz. General remarks: Ultrathin methacrylate-(e.g
Immunofluorescence on ultrathin resin sections
General remarks: Ultrathin methacrylate-(e.g.Lowicryl HM20, K4M, Monostep polar and nonpolar or LR-White) or epoxy (Epon/Araldite, Spurr) sections are transferred with a loop on poly-L-lysine coated, round cover slips
Making Boiled Donkey (or whatever) Serum (BDS) for Blocking
Mounting Media 20mM Tris pH 8.0 0.5% N-propyl gallate 90% Glycerol Store at 4oC PHEM (500 mls) 2x 18.14 g Pipes 6.5 g Hepes 3.8 g EGTA 0.99 g MgSO4 pH 7.0 w/ KOH PBS (5x in 500 mls) 20.45 g NaCl
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