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文献和实验. Procedure Add 1/10 volume 3 M NaAc, pH 5.2, and 2.5 volumes 100% ethanol. Mix and incubate at -20C for at least 1 hour. Centrifuge at > 12,000 x g in a microfuge for 20 minutes at 4C.
3 M sodium acetate (pH 5.2) to a final concentration of 0.3 M. Mix well. Add 2.5 volumes of ice-cold ethanol, mix, and store the solution for at least 30 minutes on ice. Recover the poly(A)+ RNA by centrifugation at 10,000g (9000
has been identified as a high-affinity receptor for the plant alkaloid ryanodine, and purified to apparent homogeneity as a 30s protein complex comprising four identical high- mol-wt polypeptide subunits of relative mol mass (M r ) ~560,000 (it1
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