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- 文献和实验
- 技术资料
- 供应商：
上海西宝
- 英文名：
Nuclear Extract Kit
- 规格：
100 rxns
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文献和实验Preparation of nuclear extract and cytoplasmic extract
an aliquot with Trypan Blue and check for >90% lysis on microsope.5.Spin at 2000 rpm for 15 minutes in J-6 to pellet nuclei.6.Save the supernatant for S100 extract (see below).7.Transfer the nuclear pellet to a glass beaker containing a stir bar and add
Largescale nuclear extract preparation
at the same speed and temperature. Save THE PELLET(S). V.Dialyze nuclear extract and measure protein 1.Resuspend each pellet in 500ml of dialysis buffer and dialize sample(s)against dialysis buffer (1:1,000 for 4h) 2 Clarify nuclear
Invitro transcription with yeast nuclear extract
In vitro transcription with yeast nuclear extract Steve Hahn Wear gloves
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