- 询价
- Abcam
- 中国/美国/德国
- xy50916-RP02
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温，避光
- 克隆性：
单克隆
- 抗体名：
Cystatin-E / CST6 / Cystatin-M / Cystatin-6抗体
Antibody Type : Rabbit Polyclonal Antibody ( Antibody Purification Platform )
抗体宿主 : Rabbit IgG
缓冲液 : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
制备方法 : Produced in rabbits immunized with purified, recombinant Mouse Cystatin-E / CST6 / Cystatin-M / Cystatin-6 (rM Cystatin-E / CST6 / Cystatin-M / Cystatin-6; Catalog#50916-M08H; NP_082899.1; Met1-Ala152). Cystatin-E / CST6 / Cystatin-M / Cystatin-6 specific IgG was purified by Mouse Cystatin-E / CST6 / Cystatin-M / Cystatin-6 affinity chromatography.
Cystatin-E / CST6 / Cystatin-M / Cystatin-6抗体 Background
Cystatin E/M, also referred to as CST6, is a member of type 2 cysteine proteinase inhibitors of the cystatin superfamily, and inhibits papain and cathepsin B. Cystatin E is a low molecular mass secreted protein existing in both a glycosylated (17 kDa) and an unglycosylated (14 kDa) form, with two characteristic intrachain disulfide bridges. Expression of cystatin M/E is found to be restricted to the epidermis, more specifically in the stratum granulosum, sweat glands, sebaceous glands, and the hair follicles. In addition to its function as a cysteine protease inhibitor, cystatin M/E also serves as a target for cross-linking by transglutaminases. Accordingly, cystatin M/E was suggested to be involved in barrier formation and maintenance. Furthermore, studies have revealed that cystatin M/E is frequently epigenetically inactivated during breast carcinogenesis, and thus be regarded as a candidate of tumour suppressor gene.
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文献和实验Clonality - X Chromosome Inactivation Assay
can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome
X2 （Chi square method）也是一种好适度测验的方法，可以用来检验我们的实验结果和理论值有无差异，其公式为： X2 =Σ（d2 /e） e: 为预期值，即理论值； d：预期值和观察值或实验值之差。例如我们做了以下两组豌豆测交：查卡方表，一对性状，自由度N=2-1，X2 =10，P2 =2，0.05 X2 值的计算（紫花（Rr）×白花（rr
人转录因子E2F1 ( E2F1 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E2F1 单抗包被于酶标板上，标准品和样品中的 E2F1与单抗结合，加入生物素化的抗人 E2F1 ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸，在 450nm 处测 OD 值，E2F1 浓度与 OD 值成正比
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