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- 中国/美国/德国
- xy80276-RP01
- 2025年07月12日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
常温，避光
- 克隆性：
单克隆
- 抗体名：
Cadherin-5 / CDH5 / VE-Cad抗体
Antibody Type : Rabbit Polyclonal Antibody ( Antibody Purification Platform )
抗体宿主 : Rabbit IgG
缓冲液 : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
制备方法 : Produced in rabbits immunized with purified, recombinant Rat Cadherin-5 / CDH5 / VE-Cad (rR Cadherin-5 / CDH5 / VE-Cad; Catalog#80276-R08H; Met1-Gln585). Total IgG was purified by Protein A affinity chromatography.
Cadherin-5 / CDH5 / VE-Cad抗体 Background
Cadherins (Calcium dependent adhesion molecules) are a class of transmembrane proteins. They play important roles in cell adhesion, ensuring that cells within tissues are bound together. They are dependent on calcium(Ca2+) ions to function. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of an HAV cell adhesion recognition sequence specific to type I cadherins. It has been observed that cells containing a specific cadherin subtype tend to cluster together to the exclusion of other types, both in cell culture and during development. Cadherin-5, also known as VE-cadherin, CDH5 and CD144, is single-pass type I membrane protein. It is expressed in endothelial tissues and brain and is involved in loss of heterozygosity events in breast and prostate cancer. CDH5 is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, CDH5 may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions.
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文献和实验载体克隆到腺病毒载体 -- 0-15天 4天 获得重组腺病毒 14-21天 17-22天 4-7天 噬斑纯化 5-10天 0-5天 -- 总计 26-45天 24-56天 10-17天 以体外连接方法为基础 (by Mizuguchi和Kay)，CLONTECH的Adeno-X™表达系统提供了一种快速、高效构建重组腺病毒的方法。只需4至7天就可以获得重组腺病毒，如图1所示。由于经过7天的培养之后HEK 293细胞会渐渐失去对培养皿的附着而扩散，导致噬斑融合，用传统方法制备重组腺病毒往往需要铺平
ChIP是一种强大的确定蛋白或者组蛋白修饰在基因组上定位的实验方法。染色质被分离出来后采用抗体与抗原的结合来判定目的蛋白是否结合在特定的DNA序列上或者判定目的蛋白结合位点在全基因组范围的分布（微阵列或DNA序列）。这种方法具有空间性与时效性。该实验设计为如何在细胞中进行ChIP实验提供了详细的步骤。1. 交联和细胞收获甲醛可以将蛋白质交联到DNA上。交联结果的好坏决定于交联时间的把握。我们建议样品交联的时间一般为2-30分钟。过度的交联会减少抗原的结合性和超声断裂的效率。抗原决定簇也会被掩盖
%乙醇中分色后再镜检。（二）方法二1. 取女性口腔上皮细胞涂片。待干燥。2. Carnoy固定液中固定15分钟。干燥。3. 5N HCl中10分钟。水洗。干燥。4. 0.2%甲苯胺兰染色2-5分钟。水洗。气干。5. 镜检。四、注意事项1. 涂片时应均匀涂成单层，取材细胞要多些。取材前应漱口。2. 镜检应选择完整、核质中颗粒均匀的细胞观察，X-小体大小约为1μm,多位于细胞核边缘。3. 统计X-小体阳性率，计数应选300-500个细胞。五、试剂1. 硫堇染液配方：①4%硫堇原液：4g硫堇粉末置研钵中以
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