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- 文献和实验
- 技术资料
- 保存条件:
常温,避光
- 克隆性:
单克隆
- 抗体名:
Lumican / LUM抗体
免疫原 : Recombinant Human Lumican / LUM protein (Catalog#11640-H08H)
Antibody Type : Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
克隆号 : 110
抗体宿主 : Rabbit IgG
缓冲液 : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
制备方法 : This antibody was obtained from a rabbit immunized with purified, recombinant Human Lumican / LUM (rh Lumican / LUM; Catalog#11640-H08H; NP_002336.1; Met1-Asn338).
Lumican / LUM抗体 Usage Guide特异性 :
Human Lumican / LUM
Direct ELISA : This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human Lumican / LUM. The detection limit for Human Lumican / LUM is approximately 0.00975 ng/well.
储存方法 : This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
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xy-0022xy MAPK-1/2丝裂原活化蛋白激酶(抗原)
xy-0223xy MAPKK2丝裂原活化蛋白激酶激酶
xy-0101xy M2-PK ( pyruvate Kinase M2)丙酮酸激酶-M2(抗原)
xy-0102xy M2-PK ( pyruvate Kinase M2 )丙酮酸激酶-M2(抗原)
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xy-0051xy Melan-A/MART-1黑色素-A(抗原)
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xy-0857xy Beta-arrestin 1/2(beta-arrestin 1/2)β-抑制蛋白1/2抗原
xy-0801xy ARIP2B(Activin receptor 2B)激活素受体2B/激活素受体相互作用蛋白2b抗体
xy-0027xy MTR-1A (Melatonin receptor-1A)褪黑素受体(抗原)
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xy-11018xy Apelin36脂肪炎症因子Apelin36
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xy-1155xy ASCL1/MASH1(Achaete-scute homolog 1)神经母细胞特异性转移因子抗原
xy-1282xy ASPP1(apoptosis stimulating protein of P53 1)p53凋亡刺激蛋白1抗原
xy-1283xy ASPP2(apoptosis stimulating protein of P53 2)P53凋亡刺激蛋白2抗原
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xy-2097xy AT1R/AGTR1(Angiotensin II Type 1 Receptor)血管紧张素Ⅱ-1型受体抗原
xy-0074xy NIK,NF-kappaB-Inducing KinaseNFkB诱导的激酶(抗原)
xy-0517xy ATF1 (Activating Transcription Factor1)活化复制因子1
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文献和实验Lumican Promotes Corneal Epithelial Wound Healing
fibrillogenesis, e.g., modulation of epithelial cell adhesion or migration. Healing of a corneal epithelial injury in lumican knockout (Lum–/– ) mice was significantly delayed compared with Lum+/– mice. Addition of purified lumican to cultured medium promoted
脾细胞与小鼠骨髓瘤细胞(P3-X63/Ag8)融合,融合的细胞既获得了亲代脾细胞分泌特异性抗体的特性,又具有骨髓瘤细胞大量繁殖的能力,成为一种既能分泌特异性抗体又能长命的杂交瘤细胞。该技术为抗体的分子生物学研究提供了全新的手段。极大地促进了免疫学,遗传学,分子生物学的快速发展。 但传统抗体目前也面临诸多问题,如: 每使用一管新抗体都需要进行滴定测试; 多种同型对照,实验设计繁琐; 需要添加Fc阻断试剂; 抗体不佳,阳性细胞群不明显; 抗体作为细胞生物学和生物化学中最重要的试剂之一,科学界每年在这类
人转录因子E2F1 ( E2F1 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E2F1 单抗包被于酶标板上,标准品和样品中的 E2F1与单抗结合,加入生物素化的抗人 E2F1 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450nm 处测 OD 值,E2F1 浓度与 OD 值成正比
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