- ¥1725
- pepro
- 500-P39
- 2025年07月15日
- WB ELISA Neutralization
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- 克隆性：
多克隆
- 适应物种：
人
- 宿主：
兔
- 应用范围：
WB ELISA Neutralization
- 抗体英文名：
Anti-Human LIF
- 抗体名：
Anti-Human LIF
- 规格：
50ug
Anti-Human LIF
Catalog Number:
500-P39
Source:
Polyclonal Rabbit
Preparation:
Produced from sera of rabbits immunized with highly pure recombinant Human LIF. Anti-Human LIF specific antibody was purified by affinity chromatography employing an immobilized Human LIF matrix.
Immunogen:
E.coli derived Recombinant Human LIF (PeproTech catalog #300-05)
Application:
ELISA
Sandwich
To detect Human LIF by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human LIF (500-P39Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human LIF.
To detect Human LIF by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human LIF (500-P39Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human LIF.
Western Blot
To detect Human LIF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human LIF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralization
To yield one-half maximal inhibition [ND50] of the biological activity of Human LIF (1 ng/ml), a concentration of < 0.10 μg/ml of this antibody is required. E.coli derived Recombinant Human LIF (PeproTech catalog #300-05)
Synonyms:
Leukemia Inhibitory Factor, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor (MLPLI)
Description:
LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Human LIF is a 19.6 kDa protein containing 180 amino acids residues, including three disulfide bonds.
AA Sequence:
SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
Source:
E.coli
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
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Anti-Human LIF
¥1725
