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文献和实验(7)恒温干燥箱 (8)冰箱 (9)试管架 (10)胶头滴管 (11)具塞刻度试管 20mL×24 (12)移液管或加液器 0.5 mL×3;2mL×7 (13)容量瓶 100 mL×6;1000 mL×3 (14)量筒 50 mL×2;100 mL×1;500 mL×1 (15)烧杯 100 mL×6;500mL×3;1 000 mL×1
for 14 days at 37°C. 11. Between 3 and 7 days and 10 and 14 days incubation, subculture 0.1 ml of test broth ontoan agar plate and incubate plate anaerobically as above. 12. Observe agar plates after 14 days incubation at x300 magnification using
Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction
and dissolve the precipitate by gentle inversion. 10. Add 4 ml of 4 M Lithium Acetate and incubate on ice for 20 min. 11. Centrifuge at 1,000 X g for 10 min. 12. Transfer the supernatant to a fresh tube, add 16 ml of 100% Ethanol, and incubate
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