FITC标记小鼠抗人IFN-a抗体 | Mouse Anti

Dual- and Triple-Color Fluorospot

antibodies (mAbs) to cytokines: Mouse mAb to human IFN-γ; mAb 1-D1K for coating and FITC-labeled mAb 7-B6-1 for detection. Mouse mAb to human IL-2; mAbs IL2-I/249 for coating and biotinylated mAb IL2-II for detection. If a third cytokine is included

Th17 Polarization of Mouse CD4 Cells

of anti-mouse CD28, clone 37.51(5μg/mL),IL-6 (50ng/mL),TGF-β1 (1ng/mL), anti-mouse IL-4 (10μg/mL), anti-mouse IFN-γ (10μg/mL), and 300 nM of FICZ. Alternatively, mouse IL-23 (5ng/ ml) can be used in place of FICZ. 3)        On day

Elabscience|巨噬细胞极化总失败？RAW264.7/THP-1/BMDM/hMDM四大模型避坑指南与实操全攻略

检测。 M2极化：弃去旧培养基，换为无血清培养基饥饿处理16-20 h；再更换为新鲜全培养基静息培养24 h；最后加入IL-4和IL-13（50-100 ng/mL）诱导培养24-48 h，完成M2极化。 流式检测： （1）收集细胞悬液，150 × g离心3 min； （2）弃上清，加入Cell Staining Buffer或PBS缓冲液重悬细胞沉淀，调整细胞密度为1-10×106/mL; （3）取100 μL细胞悬液，每组加入2 μL Purified Anti-Mouse CD