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文献和实验Crystallization of Kinesin Family Motor Proteins
Tris pH 7.9, 5 mM MgCl2, 0.5 mM ADPReservoir: 17.5% PEGMME 2000, 100 mM HEPES pH 6.5-7.5, 3% glycerolSong et al., 2001Kar3 +N11 (WT)Hanging Drop 18°C2 microliters of protein (15 mg/ml) + 2 microliters of well solution containing 20-26% PEG2000ME, 0.2 M
PEGPEI Copolymers for Oligonucleotide Delivery to Cells and Tissues
compounds that markedly improve the in vitro and in vivo delivery of AOs to cells and tissues. By varying the Mw of PEI, as well as the nature of PEG shielding, PEG-PEI-AO nanoparticulates can be prepared with a dynamic range of size, surface charge
Crystallization of Kinesin Family Motor Proteins
., 2001 Kar3 +N11 (WT) Hanging Drop 18°C 2 microliters of protein (15 mg/ml) + 2 microliters of well solution containing 20-26% PEG2000ME, 0.2 M NaCl, 50 mM HEPES buffer pH 7.0-8.0 Yun et al., 2001
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