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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
ETR Binding Buffer 1L
- 库存:
大量
- 供应商:
上海瑶韵生物
为了满足生物领域日益增加的要求,Omega新开发的基于磁珠的纯化方式,以其高结合力和高纯度的核酸产物等优越性,已经在欧美市场得到广泛的接收。Omega的磁珠纯化方式,与目前大部分公司采用的硅磁颗粒不同,它采用的是纳米磁珠同正离子相藕连而成的微型颗粒,该颗粒的结合能力是传统硅磁的10-30倍,因而能大大提高核酸产量和纯度,目前该产品处于世界最领先的水平。此外,为满足不同客户的需求,Omega还开发了一些成本较低的核酸纯化试剂盒,如盐析法纯化血液或组织基因组DNA纯化试剂盒,玻璃奶纯化试剂盒以及溶液型的RNA抽提试剂盒。
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文献和实验Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
58 to 0.1%.Store dilution buffer at -70 degrees.Proteins are diluted in dilution buffer and quick frozen on dry ice.Thaw proteins on ice.Proteins are typically stable to multiple repeated freeze thaw.5x binding buffer 1 ml20% glycerol 400 microliters
in 1966 (2 ). The principle of the technique is straightforward. Under a wide range of buffer conditions, protein-free nucleic acids pass freely through membrane filters, whereas proteins and their bound ligands are retained. Thus, if a particular protein
to ionic strength, which should be optimized to be high enough to prevent motor aggregation without preventing binding to microtubules. Forty to 50 mM NaCl + buffer gives good results for several motor proteins, including Ncd and Kar
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