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Phospho-MDM2(Thr218) Antibody

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  • ¥2400
  • PLLABS已认证
  • 加拿大
  • PL0304066
  • 2025年07月14日
  • WB: 1:100-500 E: 1:500-1000 IP: 1:20-100 IHC: 1:100-500 IF: 1:100-500
  • Rabbit
  • Hu, Mo, Rat, Dog, Cow, Hor
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      PhosphoPeptide derived from human MDM2 around the phosphorylation site of Thr218.

    • 亚型

      Rabbit Polyclonal IgG

    • 形态

      液态

    • 保存条件

      保存于-20℃

    • 克隆性

      多克隆

    • 标记物

      无标记(PLlabs可供应生物素、酶、荧光素标记的抗体)

    • 适应物种

      Hu, Mo, Rat, Dog, Cow, Hor

    • 保质期

      按说明书条件可保存1-2年

    • 抗原来源

      见详细说明资料

    • 目录编号

      PL0304066

    • 级别

      超纯

    • 库存

      大量

    • 供应商

      华拓生物

    • 宿主

      Rabbit

    • 应用范围

      WB: 1:100-500 E: 1:500-1000 IP: 1:20-100 IHC: 1:100-500 IF: 1:100-500

    • 浓度

      1mg/ml

    • 靶点

      Phospho-MDM2(Thr218)

    • 抗体英文名

      Phospho-MDM2(Thr218) Antibody

    • 抗体名

      磷酸化双微体2癌基因抗体

    • 规格

      0.1ml

    产品细节图片1
    Phospho-MDM2(Thr218) Antibody
    Cat#:PL0304066
    Isotype:Rabbit Polyclonal IgG
    Immunogen:PhosphoPeptide derived from human MDM2 around the phosphorylation site of Thr218.
    Purification: Immunogen affinity purified

    Storage Buffer: PBS, pH 7.4(1%BSA and 0.1% Sodium azide)

    Background:
    Inhibits TP53/p53- and TP73/p73-mediated cell cyclearrest and apoptosis by binding its transcriptional activation domain. Functions as a ubiquitin ligase E3, in the presence of E1 and E2, toward p53 and itself. Permits the nuclear export of p53 and targets it for proteasome-mediated proteolysis. Binds p53, p73, ARF(P14), ribosomal protein L5 and specifically to RNA. Can interact also with retinoblastoma protein(RB), E1A-associated protein EP300 and the E2F1 transcription factor. Forms a ternary complex with TP53/p53 and WWOX. Interacts with CDKN2AIP, MTBP, TRBG1 and USP7. Isoform Mdm2-F does not interact with TP53/p53.
    Alternate Names:
    MDM2 (phospho T218); MDM2 (phospho Thr218); p-MDM2 (T218); p-MDM2 (Thr218); Double minute 2 protein; Hdm 2; HDM2; MDM 2; Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa; MDM2BP; Mouse Double Minute 2; MTBP; Murine Double Minute Chromosome 2; Oncoprotein Mdm2; p53 Binding Protein Mdm2; Ubiquitin protein ligase E3 Mdm2; MDM2_HUMAN; E3 ubiquitin-protein ligase Mdm2.
    Specificity Reaction:Hu, Mo, Rat, Dog, Cow, Hor
    Application Notes:WB: 1:100-500 E: 1:500-1000 IP: 1:20-100 IHC: 1:100-500 IF: 1:100-500
    Storage :
    Store at –20ºC for one year. Avoid repeated freeze-thaw cycles. At 4ºC for 2-3 weeks after reconstituted.

    This product for research use only, not for use in humans, therapeutic or diagnostic procedures.
    PLlabs公司可供应相应抗体的生物素、辣根过氧化物酶、荧光素(FITC、PE、 CY3、 CY5、 CY7、 PE- CY3/5/7)标记抗体,欢迎大家咨询订购。

    产品细节图片2 产品细节图片3 产品细节图片4
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    图标文献和实验
    相关实验
    • Using Phospho‐Motif Antibodies to Determine Kinase Substrates

      comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available

    • Flow Cytometric Analysis of MDM2-Mediated Growth Arrest

      synthesis or repair can be detected in partially denatured DNA with a BrdU-specific fluorescent antibody. Subsequent staining of transfected MDM2 with a different fluorochrome provides information about whether transfected cells make significant progression

    • Optimized Protocol to Make Phospho-Specific Antibodies that Work

      , not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing

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