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DPOLA/DNA polymerase alpha Ant

ibody
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  • ¥2400
  • PLLABS已认证
  • 加拿大
  • PL0300432
  • 2025年07月14日
  • WB: 1:100-500 E: 1:500-1000 IP: 1:20-100 IHC: 1:100-500 IF: 1:100-500
  • Rabbit
  • Hu, Mo, Rat, Chi, Pig, Cow,
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      PhosphoPeptide derived from Mo TCF3 around the phosphorylation site of Thr355.

    • 亚型

      Rabbit Polyclonal IgG

    • 形态

      液态

    • 保存条件

      保存于-20℃

    • 克隆性

      多克隆

    • 标记物

      无标记(PLlabs可供应生物素、酶、荧光素标记的抗体)

    • 适应物种

      Hu, Mo, Rat, Chi, Pig, Cow,

    • 保质期

      按说明书条件可保存1-2年

    • 抗原来源

      见详细说明资料

    • 目录编号

      PL0300432

    • 级别

      超纯

    • 库存

      大量

    • 供应商

      华拓生物

    • 宿主

      Rabbit

    • 应用范围

      WB: 1:100-500 E: 1:500-1000 IP: 1:20-100 IHC: 1:100-500 IF: 1:100-500

    • 浓度

      1mg/ml

    • 靶点

      DPOLA/DNA polymerase alpha

    • 抗体英文名

      DPOLA/DNA polymerase alpha Antibody

    • 抗体名

      DNA聚合酶α抗体

    • 规格

      0.1ml

    产品细节图片1
    DPOLA/DNA polymerase alpha Antibody
    Cat#:PL0300432
    Isotype:Rabbit Polyclonal IgG
    Immunogen:PhosphoPeptide derived from Mo TCF3 around the phosphorylation site of Thr355.
    Purification: Immunogen affinity purified

    Storage Buffer: PBS, pH 7.4(1%BSA and 0.1% Sodium azide)

    Background:
    Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively.
    Alternate Names:
    TCF3/E2A(phospho T355); p-TCF3(phospho T355); p-E47(phospho T355); bHLHb21; Class B basic helix-loop-helix protein 21; E12; E2A; E2A immunoglobulin enhancer binding factors E12/E47; E47; Helix loop helix protein HE47; Immunoglobulin enhancer-binding factor E12/E47; immunoglobulin transcription factor 1; ITF1; Kappa E2 binding factor; Kappa-E2-binding factor; MGC129647; MGC129648; Negative vitamin D response element binding protein; TCF-3; TCF3; TFE2; TFE2_Mo; transcription factor 3; Transcription factor E2-alpha; Transcription factor ITF-1; VDIR; VDR interacting repressor.
    Specificity Reaction:Hu, Mo, Rat, Chi, Pig, Cow,
    Application Notes:WB: 1:100-500 E: 1:500-1000 IP: 1:20-100 IHC: 1:100-500 IF: 1:100-500
    Storage :
    Store at –20ºC for one year. Avoid repeated freeze-thaw cycles. At 4ºC for 2-3 weeks after reconstituted.

    This product for research use only, not for use in humans, therapeutic or diagnostic procedures.
    PLlabs公司可供应相应抗体的生物素、辣根过氧化物酶、荧光素(FITC、PE、 CY3、 CY5、 CY7、 PE- CY3/5/7)标记抗体,欢迎大家咨询订购。

    产品细节图片2 产品细节图片3 产品细节图片4
    PLLABS坚持提供高质量的产品和专业的技术服务,并提供完善的技术支持和售后服务。

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    图标文献和实验
    相关实验
    • In situ hybridisation to alpha satellite sequences (chromosome specific)

        Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA

    • Polymerase III in vitro Transcription

      Polymerase III in vitro Transcription Steve Hahn For the following reactions, use appropriate shielding and dispose of radioactive waste properly! A 20 microliter transcription reaction contains: 4.0 μl 5X Pol III transcription buffer 0.2 μl

    • PCR with expand Polymerase

      Expand High Fidelity PCR (Boehringer) Steve Hahn Last modified 12/12/99 Expand is a mixture of Taq and Pwo DNA polymerases (Boehringer). Pwo polymerase has 3' to 5' exo proofreading activity. If amplifying a 1 Kb fragment for 20

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