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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见产品说明
- 保质期:
见产品批号
- 英文名:
MagCellect Human B Cell Isolation Kit (1 Kit)
- 库存:
需确认
- 供应商:
嵘崴达
- CAS号:
见产品说明
- 规格:
1KT
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文献和实验with 5 ml of PBS and add to gradient. Centrifuge at 300 g for 40 minutes, room temperature. Collect the B cells at the interface. Wash 3 times with PBS. Suspend the SRBC-T cell pellet. Centrifuge at 300 d for 10 minutes. Aspirate all of the supe
Isolation of Untouched Human B Cells by Depletion of Non-B Cells
-bound cells with a magnet. Discard the bead-bound cells and use the remaining, untouched human B cells for any application. 实验试剂 Mixer allowing both tilting and rotation. Magnets Isolation Buffer: Ca2 and Mg2 free
Expression Cloning of Human B Cell Immunoglobulins
characterization of the human antibody repertoire on a single cell level through the generation of recombinant monoclonal antibodies from single primary human B cells of defined origin. This protocol offers a detailed description of the method starting
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