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- 英文名:
Mag-Bind Oligonucleotide Purification Kit(20 x 96)
- 库存:
电询
- 供应商:
上海古朵生物有限公司
品名: Mag-Bind Oligonucleotide Purification Kit(20 x 96)(PCR纯化试剂盒) 磁珠寡核苷酸纯化试剂盒:磁珠法纯化寡核苷酸片段 货号:M2513-02 品牌:Omega
Mag-Bind Oligonucleotide Purification Kit(20 x 96)(PCR纯化试剂盒)产品特性与优点
1.是纯化产物最可靠、最快速的试剂盒
2.操作简单,每次都能获得理想的效果
3.性价比最高
本产品仅用于科研实验,不用于临床诊断!
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文献和实验A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
5X Buffer, 48 ml 25% acrylamide, 500 m l 10% APS (Gibco #15523-012), 20 m l TEMED (Sigma #T8133). • Dry down 100 m g oligonucleotide and resuspend in 50 m l Formamide Dye. • Boil for 2 minutes and hold on ice until ready to load gel. • Run the gel
Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed mutagenesis from around 30% to ~75% or greater in some cases . 1、Use PAGE to purify 20-30 micrograms
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