- ￥156 - 1600
- MedChemExpress(MCE)
- 美国
- HY-D0720
- 2025年12月05日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C, sealed storage, away from moisture and light
- 英文名：
NSC-151912; L-6868
- 库存：
货期：1-2天
- 供应商：
MedChemExpress LLC
- CAS号：
2315-97-1
- 规格：
10 mM * 1 mL/5 mg/10 mg/50 mg/100 mg/500 mg
| 规格： | 10 mM * 1 mL | 产品价格： | ￥275.0 |
|---|---|---|---|
| 规格： | 5 mg | 产品价格： | ￥156.0 |
| 规格： | 10 mg | 产品价格： | ￥250.0 |
| 规格： | 50 mg | 产品价格： | ￥500.0 |
| 规格： | 100 mg | 产品价格： | ￥900.0 |
| 规格： | 500 mg | 产品价格： | ￥1600.0 |
MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务。
Lucigenin
CAS No. : 2315-97-1
MCE 国际站：Lucigenin
产品活性：Lucigenin 是一种化学发光探针。Lucigenin 可用于检测产生的内源性超氧阴离子自由基 (O2-)。Lucigenin 对氯离子极其敏感，当 Lucigenin 与氯离子结合会导致荧光淬灭。Lucigenin 也常被用作氯离子指示剂。其荧光波长：Ex/Em=455/505 nm。
研究领域：Others | Immunology/Inflammation | NF-κB | Metabolic Enzyme/Protease
作用靶点：Fluorescent Dye | Reactive Oxygen Species
In Vitro: Preparation of Lucigenin working solution
1.1 Preparation of the stock solution
Dissolve 1 mg of Lucigenin in 0.1919 mL of DMSO to obtain 10 mM of Lucigenin.
Note: It is recommended to store the stock solution at -20 °C -80 °C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Lucigenin working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of Lucigenin working solution.
Note: Please adjust the concentration of Lucigenin working solution according to the actual situation.
Cell staining
2.1 Cell preparation.
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of Lucigenin working solution, and then incubate at room temperature for 15 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope.
相关产品：Acetylcysteine | H2DCFDA | Deferoxamine mesylate | Elesclomol | Quercetin | MitoSOX Red | BODIPY 493/503 | L-Ascorbic acid | Mito-TEMPO | Nile Red | D-Luciferin potassium | Mitoquinone mesylate | JC-1 | FITC | Propidium Iodide | CFDA-SE | L-Glutathione reduced | Trolox | FITC-Dextran (MW 4000) | Zinc Protoporphyrin | FX-11 | Dihydroethidium | DiR | Dimethyl fumarate | Calcein-AM | Diphenyleneiodonium chloride | Thiazolyl Blue | 2-NBDG
热门产品线：重组蛋白 | 化合物库 | 天然产物 | 荧光染料 | PROTAC | 同位素标记物
Trending products：Recombinant Proteins | Bioactive Screening Libraries | Natural Products | Dye Reagents | PROTAC | Isotope-Labeled Compounds
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验线粒体荧光探针大全：TMRM，Mitotracker，JC-1
with MitoTracker Green FM, the CoroNa Red Na+ indicator (C24430, C24431; Section 21.1) spontaneously localizes in the mitochondria (Figure 21.14) and may be useful for measuring intramitochondrial Na+ transients. Lucigenin The well-known chemiluminescent probe
., Bohlen, H., Welz, A., Hescheler, J., Jacobsen, S.E., and Fleischmann, B.K. 2006. Engraftment of engineered ES cell‐derived cardiomyocytes but not BM cells restores contractile function to the infarcted myocardium. J. Exp. Med. 203:2315‐2327
技术资料暂无技术资料 索取技术资料
