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- 文献和实验
- 技术资料
- 供应商：
上海西宝
- 英文名：
MAXbind™ Staining Medium
- 规格：
250 ml
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文献和实验A general protocol for staining cell for cytometry analysis
General Annexin V Staining Procedure Solutions 1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1× prior to use. 2. Propidium Iodide (PI). Prepare a 50 µg/ml stock solution of PI in 1× PBS buffer
Fluorescence Mounting Medium (Antifade)
should appear almost colorless to a slight tint of yellow. If it is an intense yellow or orange color the PPD is most likely contaminated and will have background staining. pH the mounting medium to a pH of 8.0-9.0 using the Carbonate-Bicarbonate buffer
Direct Immunofluorescence Staining cells with antibodies directly linked to fluorochromes is known as direct immunofluorescence (DIF). DIF lends itself to multicolour experiments where a cell suspension is simultaneously stained
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