BLASTICIDINE S HYDROCHLORIDE*

Guanidine Hydrochloride Purification of Proteins From SDS

Pepstatin A (2.0 mg/ml)10 ml Leupeptin (1.0 mg/ml)10 ml Aprotinin (5.0 mg/ml)(Protease Inhibitor Cocktail Sigma #P8340)Denaturation Buffer6 M guanidine hydrochloride 57.3 g G-HCl20 mM Hepes 7.9 2 ml 1M Hepes pH 7.910 mM KCl 0.074 g KCl0.5 mM DTT 100 ml 0.5 M

Guanidine Hydrochloride Purification of Proteins From SDS PAGE

(Sigma #P7626) 10 ml Pepstatin A (2.0 mg/ml) 10 ml Leupeptin (1.0 mg/ml) 10 ml Aprotinin (5.0 mg/ml) (Protease Inhibitor Cocktail Sigma #P8340) Denaturation Buffer 6 M guanidine hydrochloride 57.3 g G-HCl 20 mM Hepes 7.9 2 ml 1M Hepes pH 7.9 10 mM

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

for the siRNA targeted gene compared to the respective control(s).Part III: Detection of pRNAs by 5'biotin EF52 pull-down assay 1.A minimum of ~4.0×10 6 cells are required/sample. Twenty-four hours following the plating (~70% confluent) the cultures