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文献和实验F1 plates (total 600 F2 plates). Don't try to score plate for % transmission. Screen F2 plates for 95% non-Uncs. Pick 6 L4/YA non-Uncs TIP from F2 candidate plates. Screen F3 plates for 100% non-Uncs. The stack of six plates
a chilled, sterile pipette tip to transfer the competent cells to chilled, sterile 17 x 100-mm polypropylene tubes. Store the cells on ice. Glass tubes should not be used since they lower the efficiency of transformation by ~10-fold
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
. Shake rack thoroughly during the washing procedure. iv. Rinse slides in PBS. Add 100 µL of a solution containing 0.5 mg/mL DAB and 0.01% H2 O2 . v. To prevent overstaining, follow the appearance
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