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文献和实验Alizarin Red S (Bone) and Alcian Blue (Cartilage) Staining of Cleared Skeletons.
to alizarin red S/KOH solution, until bone is stained purple. 2-3 days. (This solution can be changed daily). Rinse in 1% KOH. ~ 3 changes several hours each. 20% Glycerol in 1% KOH 24hrs (swirl occasionally) 50% Glycerol
Kamps's Western Blotting Protocol
.It is much more quantitative,at least if used in conjunction with a Phosphorimager.It has a lower background.There is less staining of abundant proteins in the sample,and for reasons that aren't clear,protein A shows much less binding to antibodies present
Immunofluorescent Staining of Drosophila Larval Brain Tissue
, place the brains in a 0.5-mL microcentrifuge tube containing cold Schneider’s insect medium. Do not let the tissue sit in Schneider’s insect medium for >20 min. Fixation and Staining 6. Remove the Schneider’s insect medium
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