【OmpA】重组细菌外膜蛋白-A,Recombinant B

【OmpA】重组细菌外膜蛋白-A，Recombinant Bacterial Outer Membrane Protein-A，OmpA

产品名称：【OmpA】重组细菌外膜蛋白-A-Recombinant Bacterial Outer Membrane Protein-A

产品货号：PRO-571

产品规格：200ug

来源宿主：大肠杆菌中重组表达

保存建议：【OmpA】重组细菌外膜蛋白-A-Recombinant Bacterial Outer Membrane Protein-ALyophilized Bacterial Outer Membrane Protein-A although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon C between 2-7 days and for future usereconstitution OmpA should be stored at 4 below -18℃. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.Please avoid freeze-thaw cycles.

背景资料：The OmpA protein is one of the main outer-membrane proteins of a large array of Gram-negative bacteria such as A. salmonicida， Shigella dysenteriae and E. coli. OmpA’s major physiological functions include maintenance of the structural integrity and morphology of the cells and porin activity， as well as a role in conjugation and bacteriophage binding. Achromogenic atypical Aeromonas salmonicida is the causative agent of goldfish ulcer disease. Virulence of this bacterium is associated with the production of a paracrystalline outer membrane A-layer protein. The species specific structural gene for the monomeric form of A-protein was cloned into a pET-3d plasmid in order to express and produce a recombinant form of the protein in E. coli BL21（DE3）. The induced protein was isolated from inclusion bodies by a simple solubilization-renaturation procedure and purified by ion exchange chromatography on Q-Sepharose to over 95% pure monomeric protein. Recombinant A-protein was compared by biochemical， immunological and molecular methods with the A-protein isolated from atypical A. salmonicida bacterial cells by the glycine and the membrane extraction methods.

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ProSpec 是一家全球著名细胞因子蛋白生产和研发的公司，具有20多年的生产经验，专注于蛋白（重组及合成）生产研发。我们的核心产品包括：干扰素、白介素、骨蛋白、肿瘤坏死因子、成纤维生长因子、瘦素、干细胞因子、催乳素、表皮生长因子、胰岛素样生长因子、神经生长因子、集落刺激因子、激素、酶、病毒抗原以及各种重组蛋白等。领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。

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