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- 文献和实验
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- 保存条件:
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- 英文名:
Recombinant Human Ku P70/P80
- 库存:
100
- 供应商:
艾美捷科技
- 规格:
1mg///20ug///5ug
【Ku P70 / P80】重组人Ku P70 / P80蛋白,Recombinant Human Ku P70/P80,Ku P70/P80
产品名称:【Ku P70 / P80】重组人Ku P70 / P80蛋白-Recombinant Human Ku P70/P80 产品货号:PRO-397 产品规格:1mg///20ug///5ug 来源宿主:昆虫Sf9细胞中重组表达
保存建议:【Ku P70 / P80】重组人Ku P70 / P80蛋白-Recombinant Human Ku P70/P80Store at 4℃ if entire vial will be used within 2-4 weeks.Store, frozen at -20℃ for longer periods of time.Avoid multiple freeze-thaw cycles.
背景资料:Ku antigen consists of two polypeptides (70 kDa and 80 kDa; the p80 subunit is occasionally called p86). The Ku heterodimer provides DNA-binding and regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V(D)J recombination, telomere maintenance, and the regulation of specific gene transcription.Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome (up to 20% of patients) but have also been detectable in about 20 % of patients with primary pulmonary hypertension (as a complication of overlap), in 5-10 % of SLE and MCTD patients, and in up to 20 % of patients with primary Sj?gren’s syndrome.In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent.
点击:【Ku P70 / P80】重组人Ku P70 / P80蛋白-Recombinant Human Ku P70/P80查看更详细产品说明,更多应用、储存、价格、货期等信息请致电垂询艾美捷科技有限公司。更多prospec公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
ProSpec 是一家全球著名细胞因子蛋白生产和研发的公司,具有20多年的生产经验,专注于蛋白(重组及合成)生产研发。我们的核心产品包括:干扰素、白介素、骨蛋白、肿瘤坏死因子、成纤维生长因子、瘦素、干细胞因子、催乳素、表皮生长因子、胰岛素样生长因子、神经生长因子、集落刺激因子、激素、酶、病毒抗原以及各种重组蛋白等。领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。
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文献和实验after specifically interacting with its receptors. Human IL-2 is a 133-amino acid polypeptide with a molecular mass of 15-18 kDa. IL-2 signaling is mediated by a multichain receptor complex consisting of an alpha (CD25), beta (CD122), and gamma (CD132) chain. The IL
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70 or GAPDH protein expression by immunofluorescence using anti-Ku (p70) and anti-GAPDH antibodies (B) . To generate the bar graph (A) , fluorescence of a single microscopic field was quantitated using MetaMorph software (Nikon). The negative control
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, both targeting GAPDH and Ku 70, were transfected into HeLa cells at a final concentration of 50 nM each. 48 hr after transfection, cells were analyzed for Ku 70 or GAPDH protein expression by immunofluorescence using anti-Ku (p70) and anti-GAPDH antibodies (B
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