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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
Room Temperature
- 保质期：
1年
- 英文名：
Plasmid Midi Kit
- 库存：
10⁺盒
- 供应商：
上海睿安生物19121878899
- 规格：
25次/盒
Qiagen货号12143质粒中提试剂盒Plasmid Midi Kit上海睿安生物19121878899
Features
- Purity equivalent to 2 x CsCl gradient centrifugation
- High yields of plasmid DNA
- Cost-effective preparations
- LyseBlue for optimum lysis and maximum DNA yield
Product Details
QIAGEN Plasmid Kits provide gravity-flow, anion-exchange tips for purification of transfection-grade plasmid DNA. Lysate clearing and isopropanol precipitation are achieved by centrifugation.
The QIAGEN Plasmid Mega Kit (cat. no. 12181) and the QIAGEN Plasmid Giga Kit (cat. no. 12191) can be used with the QIAfilter Mega-Giga Cartridges (cat. no. 19781) as an optional protocol step for rapid clearing of bacterial lysates by filtration instead of centrifugation. See more information regarding principle below.
Performance
The QIAGEN Plasmid Kits uses gravity-flow QIAGEN anion-exchange tips for efficient purification of plasmid DNA. Up to 10 mg (giga), 2.5 mg (mega), 500 µg (maxi), 100 µg (midi), and 20 µg (mini) high-copy plasmid DNA is purified from culture (culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium). Plasmid DNA purified with QIAGEN Plasmid Kits is highly suitable for use in applications such as transfection (see figure " Transfection efficiency versus plasmid purification method"), cloning, and in vitro transcription.
Principle
The unique anion-exchange resin in QIAGEN-tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation. Prepacked QIAGEN-tips operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation. The entire QIAGEN plasmid purification system avoids the use of toxic substances such as phenol, chloroform, ethidium bromide, and CsCl, minimizing hazard both to the user and the environment.
As an optional protocol step for rapid clearing of bacterial lysates by filtration instead of centrifugation, you can use the QIAfilter Mega-Giga Cartridges (cat. no. 19781), which operate with house vacuum to efficiently clear even large volumes of bacterial lysate with minimal effort. The protocol is available in the handbook.
Procedure
With QIAGEN Plasmid Kits, bacterial lysates are cleared by centrifugation. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and pure plasmid DNA is eluted in high-salt buffer (see flowchart " QIAGEN Plasmid Kit procedures"). The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation.
Applications
Plasmid DNA purified with QIAGEN Plasmid Kits is highly suitable for use in applications, such as:
- Transfection
- Cloning
- PCR
- In vitro transcription
QIAGEN是全球领先的样本和分析技术的供应者，提供各种样本纯化和分析的方法和产品。先进的、高质量的产品和服务确保了从样本获得分析结果。
纯化最高产量可达100ug的转染级别纯度的质粒或科斯质粒DNA
纯度相当于2倍氯化铯梯度离心 • 高重现性的产量和转染级别纯度
经济实惠
使用LyseBlue，获得最佳的裂解和最大的DNA产量
产品描述
QIAGEN PlasmidKits应用重力流阴离子交换柱纯化转染级纯质粒DNA。该试剂盒可制备多至20 μg （Mini）、100 μg （Midi）、500 μg（Maxi）、2.5 mg （Mega）或10 mg （Giga）的质粒DNA。离心法进行裂解液澄清和异丙醇沉淀。
订购信息:
| 货号 | 产品名称 | 产品说明 |
| 69506 | DNeasy Blood & Tissue Kit (250) | 血液，组织DNA提取试剂盒 |
| 69504 | DNeasy Blood & Tissue Kit (50) | 血液，组织DNA提取试剂盒 |
| 69104 | DNeasy Plant Mini Kit (50) | 从植物细胞和组织或真菌样本中纯化总DNA |
| 12362 | EndoFree Plasmid Maxi Kit (10) | 质粒大提试剂盒 |
| 12381 | EndoFree Plasmid Mega Kit (5) | 质粒大提试剂盒 |
| 59104 | EpiTect Bisulfite Kit (48) | 甲基化转化试剂盒 |
| 28606 | MinElute Gel Extraction Kit (250) | 胶体DNA回收试剂盒 |
| 217004 | miRNeasy Mini Kit (50) | microRNA提取试剂盒 |
| 30210 | Ni-NTA Agarose (25 ml) | 6His蛋白纯化柱料 |
| 205111 | Omniscript RT Kit (50) | 反转录试剂盒 |
| 51106 | QIAamp DNA Blood Mini Kit (250) | 血液DNA提取试剂盒 |
| 51104 | QIAamp DNA Blood Mini Kit (50) | 血液DNA提取试剂盒 |
| 51304 | QIAamp DNA Mini Kit (50) | 人类组织和细胞DNA提取试剂盒 |
| 51504 | QIAamp DNA Stool Mini Kit (50) | 人类组织和细胞DNA提取试剂盒 |
| 20021 | QIAEX II Gel Extraction Kit (150) | 胶回收纯化DNA |
| 12143 | QIAGEN Plasmid Midi Kit (25) | 质粒中提试剂盒 |
| 27104 | QIAprep Spin Miniprep Kit (50) | 提取高纯度质粒DNA |
| 28704 | QIAquick Gel Extraction Kit (50) | 胶回收试剂盒 |
| 28104 | QIAquick PCR Purification Kit (50) | PCR回收试剂盒 |
| 37900 | Qproteome Bacterial Protein Prep Kit | 细菌蛋白制备试剂盒 |
| 76106 | RNAlater (250ml) | RNAlater保护液 |
| 76104 | RNAlater (50ml) | RNAlater保护液 |
| 79254 | RNase-Free DNase Set (50) | DNA降解酶 |
| 74106 | RNeasy Mini Kit (250) | 细胞组织RNA提取试剂盒 |
| 74104 | RNeasy Mini Kit (50) | 细胞组织RNA提取试剂盒 |
| 74904 | RNeasy Plant Mini Kit (50) | 细胞组织RNA提取试剂盒 |
睿安公司专业技术服务
荧光定量PCR、甲基化检测、文库构建、原核和真核蛋白表达、高通量测序、抗体定制服务、WB检测、IHC检测。
睿安公司代理及优势品牌：
免疫学：Abgent、CST、R&D、Abcam、BD、Epitomics、Santa cruz、Jackson
耗材类： Ammex、Fisher、Bioplastics、Axygen、Corning、Greiner，Nunc-Nalgene
分子和细胞：Fermentas、hyclone-pierce、Qiagen、Bio-Rad
综合类： Sigma、 MERCK、Millipore、Roche、Invitrogen
自主品牌：Rainbio 生化类：Sigma-Vetec 、 MP
肥胖代谢类：Biovendor、ALPCO
表观遗传：Zymo ACTIVE MOTIF 神经和信号传导：Tocris
其他类： Biovision、Biocolor、Covance、Cytoskeleton、ebioscience、GE、Genetex、Invivogen、Omega、
Proteintech 、Socorex 、Vector
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- 作者
- 内容
- 询问日期
文献和实验Qiagen货号12143质粒中提试剂盒Plasmid Midi Kit上海睿安生物19121878899
Features
- Purity equivalent to 2 x CsCl gradient centrifugation
- High yields of plasmid DNA
- Cost-effective preparations
- LyseBlue for optimum lysis and maximum DNA yield
Product Details
QIAGEN Plasmid Kits provide gravity-flow, anion-exchange tips for purification of transfection-grade plasmid DNA. Lysate clearing and isopropanol precipitation are achieved by centrifugation.
The QIAGEN Plasmid Mega Kit (cat. no. 12181) and the QIAGEN Plasmid Giga Kit (cat. no. 12191) can be used with the QIAfilter Mega-Giga Cartridges (cat. no. 19781) as an optional protocol step for rapid clearing of bacterial lysates by filtration instead of centrifugation. See more information regarding principle below.
Performance
The QIAGEN Plasmid Kits uses gravity-flow QIAGEN anion-exchange tips for efficient purification of plasmid DNA. Up to 10 mg (giga), 2.5 mg (mega), 500 µg (maxi), 100 µg (midi), and 20 µg (mini) high-copy plasmid DNA is purified from culture (culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium). Plasmid DNA purified with QIAGEN Plasmid Kits is highly suitable for use in applications such as transfection (see figure " Transfection efficiency versus plasmid purification method"), cloning, and in vitro transcription.
Principle
The unique anion-exchange resin in QIAGEN-tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation. Prepacked QIAGEN-tips operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation. The entire QIAGEN plasmid purification system avoids the use of toxic substances such as phenol, chloroform, ethidium bromide, and CsCl, minimizing hazard both to the user and the environment.
As an optional protocol step for rapid clearing of bacterial lysates by filtration instead of centrifugation, you can use the QIAfilter Mega-Giga Cartridges (cat. no. 19781), which operate with house vacuum to efficiently clear even large volumes of bacterial lysate with minimal effort. The protocol is available in the handbook.
Procedure
With QIAGEN Plasmid Kits, bacterial lysates are cleared by centrifugation. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and pure plasmid DNA is eluted in high-salt buffer (see flowchart " QIAGEN Plasmid Kit procedures"). The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation.
Applications
Plasmid DNA purified with QIAGEN Plasmid Kits is highly suitable for use in applications, such as:
- Transfection
- Cloning
- PCR
- In vitro transcription
QIAGEN是全球领先的样本和分析技术的供应者，提供各种样本纯化和分析的方法和产品。先进的、高质量的产品和服务确保了从样本获得分析结果。
纯化最高产量可达100ug的转染级别纯度的质粒或科斯质粒DNA
纯度相当于2倍氯化铯梯度离心 • 高重现性的产量和转染级别纯度
经济实惠
使用LyseBlue，获得最佳的裂解和最大的DNA产量
产品描述
QIAGEN PlasmidKits应用重力流阴离子交换柱纯化转染级纯质粒DNA。该试剂盒可制备多至20 μg （Mini）、100 μg （Midi）、500 μg（Maxi）、2.5 mg （Mega）或10 mg （Giga）的质粒DNA。离心法进行裂解液澄清和异丙醇沉淀。
订购信息:
| 货号 | 产品名称 | 产品说明 |
| 69506 | DNeasy Blood & Tissue Kit (250) | 血液，组织DNA提取试剂盒 |
| 69504 | DNeasy Blood & Tissue Kit (50) | 血液，组织DNA提取试剂盒 |
| 69104 | DNeasy Plant Mini Kit (50) | 从植物细胞和组织或真菌样本中纯化总DNA |
| 12362 | EndoFree Plasmid Maxi Kit (10) | 质粒大提试剂盒 |
| 12381 | EndoFree Plasmid Mega Kit (5) | 质粒大提试剂盒 |
| 59104 | EpiTect Bisulfite Kit (48) | 甲基化转化试剂盒 |
| 28606 | MinElute Gel Extraction Kit (250) | 胶体DNA回收试剂盒 |
| 217004 | miRNeasy Mini Kit (50) | microRNA提取试剂盒 |
| 30210 | Ni-NTA Agarose (25 ml) | 6His蛋白纯化柱料 |
| 205111 | Omniscript RT Kit (50) | 反转录试剂盒 |
| 51106 | QIAamp DNA Blood Mini Kit (250) | 血液DNA提取试剂盒 |
| 51104 | QIAamp DNA Blood Mini Kit (50) | 血液DNA提取试剂盒 |
| 51304 | QIAamp DNA Mini Kit (50) | 人类组织和细胞DNA提取试剂盒 |
| 51504 | QIAamp DNA Stool Mini Kit (50) | 人类组织和细胞DNA提取试剂盒 |
| 20021 | QIAEX II Gel Extraction Kit (150) | 胶回收纯化DNA |
| 12143 | QIAGEN Plasmid Midi Kit (25) | 质粒中提试剂盒 |
| 27104 | QIAprep Spin Miniprep Kit (50) | 提取高纯度质粒DNA |
| 28704 | QIAquick Gel Extraction Kit (50) | 胶回收试剂盒 |
| 28104 | QIAquick PCR Purification Kit (50) | PCR回收试剂盒 |
| 37900 | Qproteome Bacterial Protein Prep Kit | 细菌蛋白制备试剂盒 |
| 76106 | RNAlater (250ml) | RNAlater保护液 |
| 76104 | RNAlater (50ml) | RNAlater保护液 |
| 79254 | RNase-Free DNase Set (50) | DNA降解酶 |
| 74106 | RNeasy Mini Kit (250) | 细胞组织RNA提取试剂盒 |
| 74104 | RNeasy Mini Kit (50) | 细胞组织RNA提取试剂盒 |
| 74904 | RNeasy Plant Mini Kit (50) | 细胞组织RNA提取试剂盒 |
睿安公司专业技术服务
荧光定量PCR、甲基化检测、文库构建、原核和真核蛋白表达、高通量测序、抗体定制服务、WB检测、IHC检测。
睿安公司代理及优势品牌：
免疫学：Abgent、CST、R&D、Abcam、BD、Epitomics、Santa cruz、Jackson
耗材类： Ammex、Fisher、Bioplastics、Axygen、Corning、Greiner，Nunc-Nalgene
分子和细胞：Fermentas、hyclone-pierce、Qiagen、Bio-Rad
综合类： Sigma、 MERCK、Millipore、Roche、Invitrogen
自主品牌：Rainbio 生化类：Sigma-Vetec 、 MP
肥胖代谢类：Biovendor、ALPCO
表观遗传：Zymo ACTIVE MOTIF 神经和信号传导：Tocris
其他类： Biovision、Biocolor、Covance、Cytoskeleton、ebioscience、GE、Genetex、Invivogen、Omega、
Proteintech 、Socorex 、Vector
Qiagen MIDI protocol(QIAGEN plasmid Midi试剂盒)
质粒DNA的提纯是基因工程实验中最常用的试验方法之一，质粒DNA提纯效率和质量与其后续实验步骤（如PCR扩增、酶切、连接、转化大肠杆菌和转染真核细胞等）的成功与否有着直接的关系，因此高效快速地从细菌细胞中提纯质粒DNA 具有重要的意义，本文主要介绍了由QIAGEN plasmid Midi试剂盒方法提取质粒的方法。1. Pick a single colony from a streak plate and inoculate 25 ml of LB with appropriate
【原创】【翻译】【共享】QIANGEN Plasmid Midi and Maxi Kits 试剂盒说明书
俺开始做实验已经快两个月了，郁闷死了！！！ 郁闷的原因拟另外发帖求助。 做实验过程中自己翻译的一点说明书，贴出来方便一下后面的战友！ QIANGEN Plasmid Midi and Maxi Kits 试剂盒说明书 QIANGEN Plasmid Midi and Maxi Kits 试剂盒 最大推荐培养容量 QIAGEN-tip100 QIAGEN-tip500 高拷贝质粒 25ml 100ml 低拷贝质粒 100ml 500ml
的纯RNA。 试剂盒具有以下优点： ·迅速稳定并保护RNA，确保基因完整、基因表达信息可靠。 ·由于RNA Stabilization试剂，您可以放心地在室温下操作，方便、安全--无须液氮和干冰。 ·确保RNA不受降解--即使多次冻溶也不受影响。 ·简单、快速和可靠RNA分离--使用于所有下游分析的即用型RNA。 货号 品名（规格） 价格（RMB） 74124 RNeasy Protect Mini Kit(50) 3181.00 75152 RNeasy Protect Midi
