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- 20021
- 2025年10月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Room Temperature
- 保质期：
1年
- 英文名：
QIAEX II Gel Extraction Kit
- 库存：
10⁺盒
- 供应商：
上海睿安生物19121878899
- 规格：
150次/盒
Qiagen货号20021胶回收纯化DNA(QIAEX II Gel Extraction Kit)上海睿安生物19121878899
Features
- Efficient extraction of DNA from 40 bp to 50 kb
- Gel extraction from TAE or TBE agarose gels and polyacrylamide gels
- No sodium iodide to interfere with subsequent reactions
- No shearing of large DNA fragments
Product Details
The QIAEX II system provides a suspension of silica particles to which DNA fragments bind in the presence of chaotropic salts. QIAEX II Suspension is added to solutions or solubilized agarose gel slices and binds DNA. The particles are collected by a brief centrifugation, washed and DNA of 40 bp to 50 kb is eluted in Tris buffer or water.
Performance
Using the QIAEX II system, 10 ng to 10 µg DNA are recovered efficiently (see figure "Consistent recovery"). The versatile procedure for batch purification of gel fragments can be easily scaled up to a binding capacity of 15 µg using 30 µl QIAEX II suspension.
The QIAEX II system provides silica particles for purifying 60–95% DNA fragments (40 bp – 50 kb). A volume of 10 µl QIAEX II suspension binds up to 5 µg DNA, which is subsequently eluted in 20 µl.
Principle
Purification of DNA fragments with the QIAEX II system is based on solubilization of agarose and selective adsorption of nucleic acids onto QIAEX II silica-gel particles in the presence of chaotropic salt. QIAEX II separates DNA from salts, agarose, polyacrylamide, dyes, proteins and nucleotides without phenol extraction or ethanol precipitation. QIAEX II is effective for any type of agarose in either TAE or TBE buffers.
QIAEX II particles provide a slurry for gel extraction and ensure efficient recovery without shearing, even for large DNA fragments. Optimized buffers permit DNA recovery without sodium iodide, which is difficult to remove from DNA samples, and may affect subsequent reactions.
The solubilization and binding buffer used with the QIAEX II system contains a unique pH indicator. A simple color change indicates whether the pH of the binding mixture is optimal for efficient adsorption of DNA to QIAEX II silica particles (see figure "pH indicator dye"). The colored dye also allows easy visualization of any unsolubilized agarose in the binding mixture, ensuring complete solubilization for maximum yield.
pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.
Procedure
QIAEX II silica-gel particles are added to the solubilized gel slice, and the particles collected by a brief centrifugation step (see flowchart "QIAEX II procedure"). After washing, the pure DNA fragment is eluted in 20 µl of Tris buffer or water.
The QIAEX II system provides QIAEX II suspension together with binding and wash buffers, and a comprehensive handbook. Protocols are provided for purification of DNA from agarose gels, solutions and polyacrylamide gels.
Applications
DNA purified with the QIAEX II system can be used directly in most applications, including:
- Restriction digestion
- Labeling
- Ligation
- PCR
QIAGEN是全球领先的样本和分析技术的供应者，提供各种样本纯化和分析的方法和产品。先进的、高质量的产品和服务确保了从样本获得分析结果。
更多小规模离心柱和真空形式质粒制备，参见Plasmid Miniprep Kit 选择指南。
·实际产量取决于质粒拷贝数、插入片段大小、宿主菌、培养基和培养体积。
·培养体积取决于质粒拷贝数、插入片段大小、宿主菌和培养基。
·用于从粪便中纯化最多30 μg 基因组、细菌、病毒和寄生虫DNA
·快速纯化高品质，即用型DNA
·无有机提取或乙醇沉淀
·持续的高产量
·彻底去除污染物和下游反应抑制物
应用硅胶膜技术从新鲜或冷冻人类粪便或其他样品类型（高浓度的PCR抑制剂）中抽提多至30 μg 的基因组、细菌、病毒和寄生物DNA。独特的吸附树脂InhibitEX配合经优化的缓冲液可去除PCR抑制剂。方便的QIAamp 离心柱纯化过程只需50分钟，QIAamp DNA Stool Mini Kit 可在 QIAcube 上自动纯化DNA。
QIAGEN 独特的阴离子交换柱专用于核酸纯化，其卓越的抽提性能可确保DNA纯度相当于甚至高于两次CsCl梯度离心的产物（见图"QIAGEN Plasmid kits制备超纯的质粒DNA）。预装的QIAGEN柱子以重力流方式纯化，不会流干，将质粒制备所需的手工操作时间降至最少。整个质粒纯化系统避免了苯(běn)酚、氯仿、ethidium bromide和CsCl等有毒物质，将对用户和环境的危害降至最低。
订购信息：
| 货号 | 产品名称 | 产品说明 |
| 69506 | DNeasy Blood & Tissue Kit (250) | 血液，组织DNA提取试剂盒 |
| 69504 | DNeasy Blood & Tissue Kit (50) | 血液，组织DNA提取试剂盒 |
| 69104 | DNeasy Plant Mini Kit (50) | 从植物细胞和组织或真菌样本中纯化总DNA |
| 12362 | EndoFree Plasmid Maxi Kit (10) | 质粒大提试剂盒 |
| 12381 | EndoFree Plasmid Mega Kit (5) | 质粒大提试剂盒 |
| 59104 | EpiTect Bisulfite Kit (48) | 甲基化转化试剂盒 |
| 28606 | MinElute Gel Extraction Kit (250) | 胶体DNA回收试剂盒 |
| 217004 | miRNeasy Mini Kit (50) | microRNA提取试剂盒 |
| 30210 | Ni-NTA Agarose (25 ml) | 6His蛋白纯化柱料 |
| 205111 | Omniscript RT Kit (50) | 反转录试剂盒 |
| 51106 | QIAamp DNA Blood Mini Kit (250) | 血液DNA提取试剂盒 |
| 51104 | QIAamp DNA Blood Mini Kit (50) | 血液DNA提取试剂盒 |
| 51304 | QIAamp DNA Mini Kit (50) | 人类组织和细胞DNA提取试剂盒 |
| 51504 | QIAamp DNA Stool Mini Kit (50) | 人类组织和细胞DNA提取试剂盒 |
| 20021 | QIAEX II Gel Extraction Kit (150) | 胶回收纯化DNA |
| 12143 | QIAGEN Plasmid Midi Kit (25) | 质粒中提试剂盒 |
| 27104 | QIAprep Spin Miniprep Kit (50) | 提取高纯度质粒DNA |
| 28704 | QIAquick Gel Extraction Kit (50) | 胶回收试剂盒 |
| 28104 | QIAquick PCR Purification Kit (50) | PCR回收试剂盒 |
| 37900 | Qproteome Bacterial Protein Prep Kit | 细菌蛋白制备试剂盒 |
| 76106 | RNAlater (250ml) | RNAlater保护液 |
| 76104 | RNAlater (50ml) | RNAlater保护液 |
| 79254 | RNase-Free DNase Set (50) | DNA降解酶 |
| 74106 | RNeasy Mini Kit (250) | 细胞组织RNA提取试剂盒 |
| 74104 | RNeasy Mini Kit (50) | 细胞组织RNA提取试剂盒 |
| 74904 | RNeasy Plant Mini Kit (50) | 细胞组织RNA提取试剂盒 |
睿安公司专业技术服务
荧光定量PCR、甲基化检测、文库构建、原核和真核蛋白表达、高通量测序、抗体定制服务、WB检测、IHC检测。
睿安公司代理及优势品牌：
免疫学：Abgent、CST、R&D、Abcam、BD、Epitomics、Santa cruz、Jackson
耗材类： Ammex、Fisher、Bioplastics、Axygen、Corning、Greiner，Nunc-Nalgene
分子和细胞：Fermentas、hyclone-pierce、Qiagen、Bio-Rad
综合类： Sigma、 MERCK、Millipore、Roche、Invitrogen
自主品牌：Rainbio 生化类：Sigma-Vetec 、 MP
肥胖代谢类：Biovendor、ALPCO
表观遗传：Zymo ACTIVE MOTIF 神经和信号传导：Tocris
其他类： Biovision、Biocolor、Covance、Cytoskeleton、ebioscience、GE、Genetex、Invivogen、Omega、
Proteintech 、Socorex 、Vector
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Qiagen货号20021胶回收纯化DNA(QIAEX II Gel Extraction Kit)上海睿安生物19121878899
Features
- Efficient extraction of DNA from 40 bp to 50 kb
- Gel extraction from TAE or TBE agarose gels and polyacrylamide gels
- No sodium iodide to interfere with subsequent reactions
- No shearing of large DNA fragments
Product Details
The QIAEX II system provides a suspension of silica particles to which DNA fragments bind in the presence of chaotropic salts. QIAEX II Suspension is added to solutions or solubilized agarose gel slices and binds DNA. The particles are collected by a brief centrifugation, washed and DNA of 40 bp to 50 kb is eluted in Tris buffer or water.
Performance
Using the QIAEX II system, 10 ng to 10 µg DNA are recovered efficiently (see figure "Consistent recovery"). The versatile procedure for batch purification of gel fragments can be easily scaled up to a binding capacity of 15 µg using 30 µl QIAEX II suspension.
The QIAEX II system provides silica particles for purifying 60–95% DNA fragments (40 bp – 50 kb). A volume of 10 µl QIAEX II suspension binds up to 5 µg DNA, which is subsequently eluted in 20 µl.
Principle
Purification of DNA fragments with the QIAEX II system is based on solubilization of agarose and selective adsorption of nucleic acids onto QIAEX II silica-gel particles in the presence of chaotropic salt. QIAEX II separates DNA from salts, agarose, polyacrylamide, dyes, proteins and nucleotides without phenol extraction or ethanol precipitation. QIAEX II is effective for any type of agarose in either TAE or TBE buffers.
QIAEX II particles provide a slurry for gel extraction and ensure efficient recovery without shearing, even for large DNA fragments. Optimized buffers permit DNA recovery without sodium iodide, which is difficult to remove from DNA samples, and may affect subsequent reactions.
The solubilization and binding buffer used with the QIAEX II system contains a unique pH indicator. A simple color change indicates whether the pH of the binding mixture is optimal for efficient adsorption of DNA to QIAEX II silica particles (see figure "pH indicator dye"). The colored dye also allows easy visualization of any unsolubilized agarose in the binding mixture, ensuring complete solubilization for maximum yield.
pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.
Procedure
QIAEX II silica-gel particles are added to the solubilized gel slice, and the particles collected by a brief centrifugation step (see flowchart "QIAEX II procedure"). After washing, the pure DNA fragment is eluted in 20 µl of Tris buffer or water.
The QIAEX II system provides QIAEX II suspension together with binding and wash buffers, and a comprehensive handbook. Protocols are provided for purification of DNA from agarose gels, solutions and polyacrylamide gels.
Applications
DNA purified with the QIAEX II system can be used directly in most applications, including:
- Restriction digestion
- Labeling
- Ligation
- PCR
QIAGEN是全球领先的样本和分析技术的供应者，提供各种样本纯化和分析的方法和产品。先进的、高质量的产品和服务确保了从样本获得分析结果。
更多小规模离心柱和真空形式质粒制备，参见Plasmid Miniprep Kit 选择指南。
·实际产量取决于质粒拷贝数、插入片段大小、宿主菌、培养基和培养体积。
·培养体积取决于质粒拷贝数、插入片段大小、宿主菌和培养基。
·用于从粪便中纯化最多30 μg 基因组、细菌、病毒和寄生虫DNA
·快速纯化高品质，即用型DNA
·无有机提取或乙醇沉淀
·持续的高产量
·彻底去除污染物和下游反应抑制物
应用硅胶膜技术从新鲜或冷冻人类粪便或其他样品类型（高浓度的PCR抑制剂）中抽提多至30 μg 的基因组、细菌、病毒和寄生物DNA。独特的吸附树脂InhibitEX配合经优化的缓冲液可去除PCR抑制剂。方便的QIAamp 离心柱纯化过程只需50分钟，QIAamp DNA Stool Mini Kit 可在 QIAcube 上自动纯化DNA。
QIAGEN 独特的阴离子交换柱专用于核酸纯化，其卓越的抽提性能可确保DNA纯度相当于甚至高于两次CsCl梯度离心的产物（见图"QIAGEN Plasmid kits制备超纯的质粒DNA）。预装的QIAGEN柱子以重力流方式纯化，不会流干，将质粒制备所需的手工操作时间降至最少。整个质粒纯化系统避免了苯(běn)酚、氯仿、ethidium bromide和CsCl等有毒物质，将对用户和环境的危害降至最低。
订购信息：
| 货号 | 产品名称 | 产品说明 |
| 69506 | DNeasy Blood & Tissue Kit (250) | 血液，组织DNA提取试剂盒 |
| 69504 | DNeasy Blood & Tissue Kit (50) | 血液，组织DNA提取试剂盒 |
| 69104 | DNeasy Plant Mini Kit (50) | 从植物细胞和组织或真菌样本中纯化总DNA |
| 12362 | EndoFree Plasmid Maxi Kit (10) | 质粒大提试剂盒 |
| 12381 | EndoFree Plasmid Mega Kit (5) | 质粒大提试剂盒 |
| 59104 | EpiTect Bisulfite Kit (48) | 甲基化转化试剂盒 |
| 28606 | MinElute Gel Extraction Kit (250) | 胶体DNA回收试剂盒 |
| 217004 | miRNeasy Mini Kit (50) | microRNA提取试剂盒 |
| 30210 | Ni-NTA Agarose (25 ml) | 6His蛋白纯化柱料 |
| 205111 | Omniscript RT Kit (50) | 反转录试剂盒 |
| 51106 | QIAamp DNA Blood Mini Kit (250) | 血液DNA提取试剂盒 |
| 51104 | QIAamp DNA Blood Mini Kit (50) | 血液DNA提取试剂盒 |
| 51304 | QIAamp DNA Mini Kit (50) | 人类组织和细胞DNA提取试剂盒 |
| 51504 | QIAamp DNA Stool Mini Kit (50) | 人类组织和细胞DNA提取试剂盒 |
| 20021 | QIAEX II Gel Extraction Kit (150) | 胶回收纯化DNA |
| 12143 | QIAGEN Plasmid Midi Kit (25) | 质粒中提试剂盒 |
| 27104 | QIAprep Spin Miniprep Kit (50) | 提取高纯度质粒DNA |
| 28704 | QIAquick Gel Extraction Kit (50) | 胶回收试剂盒 |
| 28104 | QIAquick PCR Purification Kit (50) | PCR回收试剂盒 |
| 37900 | Qproteome Bacterial Protein Prep Kit | 细菌蛋白制备试剂盒 |
| 76106 | RNAlater (250ml) | RNAlater保护液 |
| 76104 | RNAlater (50ml) | RNAlater保护液 |
| 79254 | RNase-Free DNase Set (50) | DNA降解酶 |
| 74106 | RNeasy Mini Kit (250) | 细胞组织RNA提取试剂盒 |
| 74104 | RNeasy Mini Kit (50) | 细胞组织RNA提取试剂盒 |
| 74904 | RNeasy Plant Mini Kit (50) | 细胞组织RNA提取试剂盒 |
