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- Qiagen
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- 69104
- 2026年03月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Room Temperature
- 保质期：
1年
- 英文名：
DNeasy Plant Mini Kit
- 库存：
10⁺盒
- 供应商：
上海睿安生物19121878899
- 规格：
50次/盒
Qiagen货号69104现货DNeasy Plant Mini Kit从植物细胞和组织或真菌样本中纯化总DNA上海睿安生物19121878899
Features
- Pure DNA, free from contaminants and enzyme inhibitors
- Rapid extraction of ready-to-use DNA
- No organic extraction, no ethanol precipitation
Product Details
The DNeasy Plant Kits provide fast and easy silica-based DNA isolation from plant samples in spin column format. Typical yields are 3–260 μg of high-quality DNA, depending on the samples used (e.g., wheat, maize, Arabidopsis, tomato, tobacco) and binding capacity of the DNeasy silica membrane. The DNeasy Plant Kits also provide silica-based DNA purification in a convenient 96-well plate format with typical yields of 1–15 μg DNA, depending on the plant species. DNA isolation from plant tissue is also automatable on the QIAcube Connect
Performance
The DNeasy Plant Kits allow rapid and efficient isolation of high-quality DNA from a wide variety of plant species and tissue types, including the most demanding sources (see table "Selection of plant species processed with DNeasy Kits"). The samples may be fresh, frozen or dried. The optimized DNeasy Plant procedure incorporates the QIAshredder spin column, a unique filtration and homogenization column that efficiently removes cell debris and improves sample handling following lysis.
Principle
The DNeasy Plant Kits use advanced silica-membrane technology and simple spin procedures to isolate highly pure total cellular DNA from plant tissues and cells or fungi. The DNeasy technology replaces cumbersome DNA isolation procedures such as CTAB, phenol or chloroform extraction. Alcohol precipitation is also not necessary, since the purified DNA is ready for immediate use.
Procedure
Samples are first mechanically disrupted and then chemically lysed (see flowchart " DNeasy Plant and DNeasy 96 Plant procedures"). RNA is removed by RNAse digestion during lysis. Cell debris, precipitated proteins, and polysaccharides are removed and the sample is homogenized by centrifugation through a QIAshredder spin column. Buffering conditions are adjusted and the lysate is loaded onto the DNeasy Plant Mini spin column. During a brief spin, DNA selectively binds to the silica membrane while contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in one or two efficient wash steps. Pure DNA is then eluted in water or low-salt buffer, ready for use.
Applications
DNeasy Plant Kits provide purification of ready-to-use DNA from plant samples, including plant cells, plant tissues and fungi.
The DNeasy Plant Kit is designed for:
- DNA extraction from plants and plant-associated microorganisms
- PCR and NGS analysis
- Marker-assisted breeding
- Plant pathogen research
- Studies on genetically modified plants
- Detection of resistance traits
Qiagen是全球领先的样本和分析技术的供应者，提供各种样本纯化和分析的方法和产品。先进的、高质量的产品和服务确保了从样本获得分析结果。
·高通量纯化血液或无细胞体液中的总DNA，包括基因组DNA，线粒体DNA，病毒DNA，最高可达60 μg总DNA
·快速纯化高品质，即用型DNA
·无有机提取或乙醇沉淀
·持续的高产量
·彻底去除污染物和下游反应抑制物
用于处理多至200 μl 的人全血， midi kits 处理量为0.3-2 ml，maxi kits为3-10 ml。QIAamp Mini、Midi 和Maxi离心管可在离心机或真空底座上制备样品。QIAamp DNA Blood Mini Kit 可在QIAcube上全自动进行DNA纯化。
订购信息：
| 货号 | 产品名称 | 产品说明 |
| 69506 | DNeasy Blood & Tissue Kit (250) | 血液，组织DNA提取试剂盒 |
| 69504 | DNeasy Blood & Tissue Kit (50) | 血液，组织DNA提取试剂盒 |
| 69104 | DNeasy Plant Mini Kit (50) | 从植物细胞和组织或真菌样本中纯化总DNA |
| 12362 | EndoFree Plasmid Maxi Kit (10) | 质粒大提试剂盒 |
| 12381 | EndoFree Plasmid Mega Kit (5) | 质粒大提试剂盒 |
| 59104 | EpiTect Bisulfite Kit (48) | 甲基化转化试剂盒 |
| 28606 | MinElute Gel Extraction Kit (250) | 胶体DNA回收试剂盒 |
| 217004 | miRNeasy Mini Kit (50) | microRNA提取试剂盒 |
| 30210 | Ni-NTA Agarose (25 ml) | 6His蛋白纯化柱料 |
| 205111 | Omniscript RT Kit (50) | 反转录试剂盒 |
| 51106 | QIAamp DNA Blood Mini Kit (250) | 血液DNA提取试剂盒 |
| 51104 | QIAamp DNA Blood Mini Kit (50) | 血液DNA提取试剂盒 |
| 51304 | QIAamp DNA Mini Kit (50) | 人类组织和细胞DNA提取试剂盒 |
| 51504 | QIAamp DNA Stool Mini Kit (50) | 人类组织和细胞DNA提取试剂盒 |
| 20021 | QIAEX II Gel Extraction Kit (150) | 胶回收纯化DNA |
| 12143 | QIAGEN Plasmid Midi Kit (25) | 质粒中提试剂盒 |
| 27104 | QIAprep Spin Miniprep Kit (50) | 提取高纯度质粒DNA |
| 28704 | QIAquick Gel Extraction Kit (50) | 胶回收试剂盒 |
| 28104 | QIAquick PCR Purification Kit (50) | PCR回收试剂盒 |
| 37900 | Qproteome Bacterial Protein Prep Kit | 细菌蛋白制备试剂盒 |
| 76106 | RNAlater (250ml) | RNAlater保护液 |
| 76104 | RNAlater (50ml) | RNAlater保护液 |
| 79254 | RNase-Free DNase Set (50) | DNA降解酶 |
| 74106 | RNeasy Mini Kit (250) | 细胞组织RNA提取试剂盒 |
| 74104 | RNeasy Mini Kit (50) | 细胞组织RNA提取试剂盒 |
| 74904 | RNeasy Plant Mini Kit (50) | 细胞组织RNA提取试剂盒 |
睿安公司专业技术服务
荧光定量PCR、甲基化检测、文库构建、原核和真核蛋白表达、高通量测序、抗体定制服务、WB检测、IHC检测。
睿安公司代理及优势品牌：
免疫学：Abgent、CST、R&D、Abcam、BD、Epitomics、Santa cruz、Jackson
耗材类： Ammex、Fisher、Bioplastics、Axygen、Corning、Greiner，Nunc-Nalgene
分子和细胞：Fermentas、hyclone-pierce、Qiagen、Bio-Rad
综合类： Sigma、 MERCK、Millipore、Roche、Invitrogen
自主品牌：Rainbio 生化类：Sigma-Vetec 、 MP
肥胖代谢类：Biovendor、ALPCO
表观遗传：Zymo ACTIVE MOTIF 神经和信号传导：Tocris
其他类： Biovision、Biocolor、Covance、Cytoskeleton、ebioscience、GE、Genetex、Invivogen、Omega、
Proteintech 、Socorex 、Vector
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文献和实验Qiagen货号69104现货DNeasy Plant Mini Kit从植物细胞和组织或真菌样本中纯化总DNA上海睿安生物19121878899
Features
- Pure DNA, free from contaminants and enzyme inhibitors
- Rapid extraction of ready-to-use DNA
- No organic extraction, no ethanol precipitation
Product Details
The DNeasy Plant Kits provide fast and easy silica-based DNA isolation from plant samples in spin column format. Typical yields are 3–260 μg of high-quality DNA, depending on the samples used (e.g., wheat, maize, Arabidopsis, tomato, tobacco) and binding capacity of the DNeasy silica membrane. The DNeasy Plant Kits also provide silica-based DNA purification in a convenient 96-well plate format with typical yields of 1–15 μg DNA, depending on the plant species. DNA isolation from plant tissue is also automatable on the QIAcube Connect
Performance
The DNeasy Plant Kits allow rapid and efficient isolation of high-quality DNA from a wide variety of plant species and tissue types, including the most demanding sources (see table "Selection of plant species processed with DNeasy Kits"). The samples may be fresh, frozen or dried. The optimized DNeasy Plant procedure incorporates the QIAshredder spin column, a unique filtration and homogenization column that efficiently removes cell debris and improves sample handling following lysis.
Principle
The DNeasy Plant Kits use advanced silica-membrane technology and simple spin procedures to isolate highly pure total cellular DNA from plant tissues and cells or fungi. The DNeasy technology replaces cumbersome DNA isolation procedures such as CTAB, phenol or chloroform extraction. Alcohol precipitation is also not necessary, since the purified DNA is ready for immediate use.
Procedure
Samples are first mechanically disrupted and then chemically lysed (see flowchart " DNeasy Plant and DNeasy 96 Plant procedures"). RNA is removed by RNAse digestion during lysis. Cell debris, precipitated proteins, and polysaccharides are removed and the sample is homogenized by centrifugation through a QIAshredder spin column. Buffering conditions are adjusted and the lysate is loaded onto the DNeasy Plant Mini spin column. During a brief spin, DNA selectively binds to the silica membrane while contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in one or two efficient wash steps. Pure DNA is then eluted in water or low-salt buffer, ready for use.
Applications
DNeasy Plant Kits provide purification of ready-to-use DNA from plant samples, including plant cells, plant tissues and fungi.
The DNeasy Plant Kit is designed for:
- DNA extraction from plants and plant-associated microorganisms
- PCR and NGS analysis
- Marker-assisted breeding
- Plant pathogen research
- Studies on genetically modified plants
- Detection of resistance traits
Qiagen是全球领先的样本和分析技术的供应者，提供各种样本纯化和分析的方法和产品。先进的、高质量的产品和服务确保了从样本获得分析结果。
·高通量纯化血液或无细胞体液中的总DNA，包括基因组DNA，线粒体DNA，病毒DNA，最高可达60 μg总DNA
·快速纯化高品质，即用型DNA
·无有机提取或乙醇沉淀
·持续的高产量
·彻底去除污染物和下游反应抑制物
用于处理多至200 μl 的人全血， midi kits 处理量为0.3-2 ml，maxi kits为3-10 ml。QIAamp Mini、Midi 和Maxi离心管可在离心机或真空底座上制备样品。QIAamp DNA Blood Mini Kit 可在QIAcube上全自动进行DNA纯化。
订购信息：
| 货号 | 产品名称 | 产品说明 |
| 69506 | DNeasy Blood & Tissue Kit (250) | 血液，组织DNA提取试剂盒 |
| 69504 | DNeasy Blood & Tissue Kit (50) | 血液，组织DNA提取试剂盒 |
| 69104 | DNeasy Plant Mini Kit (50) | 从植物细胞和组织或真菌样本中纯化总DNA |
| 12362 | EndoFree Plasmid Maxi Kit (10) | 质粒大提试剂盒 |
| 12381 | EndoFree Plasmid Mega Kit (5) | 质粒大提试剂盒 |
| 59104 | EpiTect Bisulfite Kit (48) | 甲基化转化试剂盒 |
| 28606 | MinElute Gel Extraction Kit (250) | 胶体DNA回收试剂盒 |
| 217004 | miRNeasy Mini Kit (50) | microRNA提取试剂盒 |
| 30210 | Ni-NTA Agarose (25 ml) | 6His蛋白纯化柱料 |
| 205111 | Omniscript RT Kit (50) | 反转录试剂盒 |
| 51106 | QIAamp DNA Blood Mini Kit (250) | 血液DNA提取试剂盒 |
| 51104 | QIAamp DNA Blood Mini Kit (50) | 血液DNA提取试剂盒 |
| 51304 | QIAamp DNA Mini Kit (50) | 人类组织和细胞DNA提取试剂盒 |
| 51504 | QIAamp DNA Stool Mini Kit (50) | 人类组织和细胞DNA提取试剂盒 |
| 20021 | QIAEX II Gel Extraction Kit (150) | 胶回收纯化DNA |
| 12143 | QIAGEN Plasmid Midi Kit (25) | 质粒中提试剂盒 |
| 27104 | QIAprep Spin Miniprep Kit (50) | 提取高纯度质粒DNA |
| 28704 | QIAquick Gel Extraction Kit (50) | 胶回收试剂盒 |
| 28104 | QIAquick PCR Purification Kit (50) | PCR回收试剂盒 |
| 37900 | Qproteome Bacterial Protein Prep Kit | 细菌蛋白制备试剂盒 |
| 76106 | RNAlater (250ml) | RNAlater保护液 |
| 76104 | RNAlater (50ml) | RNAlater保护液 |
| 79254 | RNase-Free DNase Set (50) | DNA降解酶 |
| 74106 | RNeasy Mini Kit (250) | 细胞组织RNA提取试剂盒 |
| 74104 | RNeasy Mini Kit (50) | 细胞组织RNA提取试剂盒 |
| 74904 | RNeasy Plant Mini Kit (50) | 细胞组织RNA提取试剂盒 |
睿安公司专业技术服务
荧光定量PCR、甲基化检测、文库构建、原核和真核蛋白表达、高通量测序、抗体定制服务、WB检测、IHC检测。
睿安公司代理及优势品牌：
免疫学：Abgent、CST、R&D、Abcam、BD、Epitomics、Santa cruz、Jackson
耗材类： Ammex、Fisher、Bioplastics、Axygen、Corning、Greiner，Nunc-Nalgene
分子和细胞：Fermentas、hyclone-pierce、Qiagen、Bio-Rad
综合类： Sigma、 MERCK、Millipore、Roche、Invitrogen
自主品牌：Rainbio 生化类：Sigma-Vetec 、 MP
肥胖代谢类：Biovendor、ALPCO
表观遗传：Zymo ACTIVE MOTIF 神经和信号传导：Tocris
其他类： Biovision、Biocolor、Covance、Cytoskeleton、ebioscience、GE、Genetex、Invivogen、Omega、
Proteintech 、Socorex 、Vector
A quick RNA mini-prep for Neurospora mycelial cultures
, during time series analyses or when screening transfor-mants for expression of a transformed gene. Under such circumstances, existing tech-niques are overly time consuming and yield more RNA than is necessary. The availability of a rapid RNA mini-prep
盒。 货号 品名（规格） 价格（RMB） 51104 QIAamp DNA Blood Mini Kit(50) 51183 QIAamp DNA Blood Midi Kit(20) 51192 QIAamp DNA Blood Maxi Kit(10) 51304 QIAamp DNA Mini Kit(50) 51504 QIAamp DNA Stool Mini Kit(50)DNeasy System 是QIAGEN公司为除临床样本以外的其他样本，如动物组织、血液、酵母、细菌、植物
） 51304 QIAamp DNA Mini Kit（50） 51504 QIAamp DNA Stool Mini Kit（50） DNeasy System 是QIAGEN公司为除临床样本以外的其他样本，如动物组织、血液、酵母、细菌、植物等快速制备基因组DNA而设计的，采用硅胶膜技术，无需酚氯仿抽提和乙醇沉淀。单个离心柱或96孔纯化板满足不同的需求。 货号 品名（规格） 价格（RMB） 69504 DNeasy Tissue Kit（50） 69103 DNeasy
