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- 克隆性:
多克隆
- 抗体英文名:
Phospho (Tyr1472) NMDA NR2B Antibody
- 规格:
100 ul
产品名称:Phospho (Tyr1472) NMDA NR2B抗体|Phospho (Tyr1472) NMDA NR2B Antibody
【产品形式】:艾美捷为您提供的100 ul Phospho (Tyr1472) NMDA NR2B抗体产品为无色溶液其浓度为N/A溶解于包含有50%甘油, 0.01% BSA、150 mM NaCl和10 mM HEPES缓冲液(pH 7.5)中.请将该Phospho (Tyr1472) NMDA NR2B抗体产品置于-20 °C冷冻保存,建议分装使用以避免反复冻融.
【背景资料】:The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). Recent work suggests that phosphorylation of Tyr1472 on NR2B may regulate the functional expression the receptor in LTP and other forms of plasticity (Nakazawa et al., 2001; Roche et al., 2001).
BioVision的公司总部在美丽的旧金山湾地区,它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有 卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品,以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代 谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理, 艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.
点击:Phospho (Tyr1472) NMDA NR2B抗体,查看更详细产品说明,更多应用、储存、价格、货期等信息请致电400-6800-868垂询BioVision中国区域授权总代理艾美捷科技有限公司。
关键词:Phospho ,Phospho (Tyr1472) NMDA NR2B抗体
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文献和实验运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
signaling公司的Phospho-PI3K p85 (Tyr458)/p55(Tyr199) 我自己分析没有条带无非是细胞状态不好,或者抗体不好,不知道还有没有可能是没有加磷酸化酶抑制剂,以至于磷酸化的蛋白被去磷酸化了? 谢谢啦~ yhwangcams 检测内参蛋白如GAPDH,ACTIN,TUBULIN是否成功? 用PLB裂解细胞得到的蛋白适合做REPORTER,做WESTERN不太合适。建议可以用其他BUFFER裂解蛋白。
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
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