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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
多克隆
- 抗体英文名:
cIAP-1 Antibody,API1,cIAP1,C-IAP1,HIAP-2,HIAP2,RNF48,MIHB,IAP2,BIRC2
- 规格:
100 ug
产品名称:cIAP-1抗体|cIAP-1 Antibody,API1,cIAP1,C-IAP1,HIAP-2,HIAP2,RNF48,MIHB,IAP2,BIRC2
【产品形式】:艾美捷为您提供的cIAP-1抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该cIAP-1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.
【背景资料】:Apoptosis can be inhibited by a group of proteins called inhibitors of apoptosis (IAPs). These proteins contain a BIR (baculovirus IAP repeat) domain near the amino-terminus. The BIR domain can bind some caspases. Many members of the IAP family of proteins block proteolytic activation of caspase-3 and –7. For example, XIAP, cIAP-1 and cIAP-2 appear to block cytochrome c-induced activation of caspase-9, thereby preventing initiation of the caspase cascade. Since cIAP-1 and cIAP-2 were first identified as components in the cytosolic death domain-induced complex associated with the TNF family of receptors, they may inhibit apoptosis by additional mechanisms.
BioVision的公司总部在美丽的旧金山湾地区,它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有 卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品,以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代 谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理, 艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.
点击:cIAP-1抗体,查看更详细产品说明,更多应用、储存、价格、货期等信息请致电400-6800-868垂询BioVision中国区域授权总代理艾美捷科技有限公司。
关键词:cIAP,cIAP-1抗体
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文献和实验. The idea behind using the same restriction enzymes is to create complementary sticky ends, which will facilitate ligation later on. A phosphatase (commonly Calf Intestinal Alkaline Phosphatase; CIAP) is also added to prevent self-ligation of the destination
of 5´-ends) 40μl CIAP 10X Reaction Buffer 5μl Diluted CIAP (0.01u/μl) up to 5μl 50μl 3. Incubate at 37°C for 30 minutes. 4. Add another aliquot of diluted CIAP (equivalent to the amount used in Step 2), and continue incubation at 37°C for an additional
15分钟以上; 4.12000g 4℃冷冻离心15分钟; 5.倒去上清,用75%乙醇浸洗沉淀,风干后外源DNA溶于10 ml ddH2O(0.5ml tube中),PUC18溶于20 ml ddH2O(1.5ml tube中); 6.按以下反应去除载体PUC18的5’磷酸基团,50℃反应30 min 以上 DNA 20 ml CIAP(TaKaRa) 0.5 ml 10 ´ buffer 4.0 ml
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