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文献和实验Clonality - X Chromosome Inactivation Assay
can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
1.5 ml 1.2 mM EDTA 0.5M stock 120 µl 1.1% Triton X-100 10% stock 5.5 ml 0.01% SDS 10% stock 50 µl
E. Z. N.A.TM X-press Plasmid Protocol
at maximum speed (13,000 x g) for 1 minute to dry the column. 14. Transfer the X-press spin column into a new 1.5 ml centrifuge tube. Add 50 uL Elution Buffer (10mM Tris Hcl pH 8.5) directly onto the center of the membrane. 15. Centrifuge
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