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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10 µg
- 供应商：
西宝生物
- 保存条件：
0 C
- 英文名：
cDNA PNEF-FS EXPRESSED IN PLASMID pBC-12/CMV
- 规格：
BIOCHEMICALS
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文献和实验at the 5' end of the original mRNA strand. Insertion of cDNA into plasmid. To complete our construction of a useful cDNA library we need a way to maintain and propagate our cDNA. We can accomplish this by inserting the cDNA
the generation of normalized, full?length?enriched cDNA libraries, and enabling efficient RNA analyses. Curr. Protoc. Mol. Biol. 90:5.12.1?5.12.27. © 2010 by John Wiley & Sons, Inc. Keywords: cDNA normalization; duplex?specific nuclease (DSN
A method for the absolute quantification of cDNA using real
and a plasmid reference curve. PicoGreen and reference standards are used to measure the amount of cDNA present in a sample using fluorescence. Real-time PCR products are cloned into plasmids and then used to calibrate unknown samples. This cloning is achieved
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