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- 英文名：
YEAST LYTIC ENZYME
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文献和实验Bacteria and Yeast Cell Disruption Using Lytic Enzymes
Enzymatic methods provide a convenient alternative for overcoming technical disadvantages of mechanical disruption. Protocols for protein extraction from bacteria and Saccharomyces cerevisiae using lytic enzymes are presented in this chapter
Isolation of Covalent Enzyme-DNA Complexes
present in the active site of the enzyme. A novel aspect of the breakagereunion reaction is the the formation of a transient covalent O 4 -phosphotyrosyl bond between the topoisomerase protein and a phosphate residue present at the break site in the DNA
yeast plasmid (1 ). The method consists of the insertion of specific recombination sites from a yeast plasmid with the aid of a yeast integrative vector (YIp) and the introduction of another plasmid carrying the gene for the corresponding site-specific
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