Nla III5′...CATG/...3′

miRNA的靶基因的寻找与鉴定

信息学的方式进行，主要是根据以下几个方面进行预测：①miRNA与靶基因结合位点的互补性；②miRNA靶位点在不同物种之间的保守性；③miRNA-mRNA结合的热稳定性；④miRNA靶位点处不应有复杂二级结构；⑤miRNA5′端与靶基因的结合能力强于3′端。除以上几个基本原则外，不同的预测方法还会根据各自总结的规律对算法进行不同的限制与优化。目前比较常用的靶基因预测软件有：miRanda、TargetScan、PicTar、miRWalk、miRanda、miRDB等，其中在miRWalk中可以查找到已经

Quantification of Gene Transcripts with Deep Sequencing Analysis of Gene Expression (DSAGE) Using 1 to 2 µg Total RNA

Figure 25.B0.4 Tags aligned to the Nkx2‐5 locus from mouse left ventricle. Nkx2‐5 has three Nla III sites found in the first exon (denoted by an asterisk). The majority of the sequence tags were aligned to the most 3′ Nla III site

Generation of Longer cDNA Fragments from SAGE Tags for Gene Identification

). In this high-throughput GLGI procedure, 3′ cDNAs starting from the last CATG are used as the templates for GLGI amplification, a SAGE tag sequence is used as the sense primer, and a universal sequence located at the 3′ end of all the cDNA templates generated