Lysyl Endopeptidase;Achromobacter protase)From Achromobacter lyticus M497-1. Supplied as a lyophilized, white powder, free of salts. Each vial contains 10 units, in 1ml Tris-HCl, 2mM, pH 8.0.Activity: <126>4 units/mgCatalyzes cleavage of the peptide bonds at the carboxy-terminal of lysine residues.Unit Definition: The amount of enzyme required for the formation of 1 mmol of p-nitroaniline per minute at 30<144>C pH 9.5.Ref.: 1. Masaki, T., Nakamura, K., Isono, M. and Soejima, M., Agricultural and Biological Chemistry, 42: 1443-5 (1978). 2. Masaki, T., Fujihashi, T., Nakamura, K. and Soejima, M., Biochem. Biophys. Acta., 660: 44 (1981). 3. Masaki, T., Fujihashi, T., Nakamura, K. and Soejima, M., Biochem. Biophys. Acta., 660: 51 (1981). 4. Morihara, K., Oka, T., Tsuzaki, H., Tochino, Y. and Kanaya, T., Biochemical and Biophysical Research Communications, 92: 396-402 (1980). 5. Masaki, T. and Soejima, M., Agricultural and Biological Chemistry, 49: 1867-8 (1985). 6. Makano, K., Tashiro, Y., Kikumoto, Y., Tagaya, M. and Fukui, T., The Journal of Biological Chemistry, 261: 8224-28 (1986).