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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
L-asparagine
- 供应商:
艾美捷科技有限公司
- 规格:
10g
【产品形式】:Autophagy inhibitor (autophagosome - lysosome fusion)
【背景资料】:Inhibitor of autophagosome-lysosome fusion.
英国Viva Bioscience公司是一家研发、生产并销售蛋白降解相关产品的公司。其产品主要用于细胞自噬、泛素化和蛋白酶体研究(也覆盖其他蛋白降解相关的产品),其产品价格合理、质量可靠且具有创新性。目前VIVA Bioscience提供逾460种产品,涉及细胞自噬、蛋白泛素化和讲解相关的所有试剂盒、蛋白、底物以及多种抑制剂、激活剂等全系列产品。作为多家进口品牌的中国区域总代理,艾美捷科技与Viva Bioscience一道为中国科研工作者们提供更好、更新的自噬、泛素化和蛋白酶体等研究工具。
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文献和实验Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase
utilizes the determination of ammonia liberated from L-asparagine in the enzyme reaction by the Nessler reaction. L-asparaginase catalyzes the hydrolysis of L-asparagine to L-aspartic acid and ammonia. Start the reaction by adding 0.1 ml
Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase
. Prepare cell suspensions also in distilled water or 0.05 M KPi with no hexane as controls. Measure the enzyme activity utilizing the Nesslerization reaction. This method utilizes the determination of ammonia liberated from L-asparagine
Extraction of DNA from Schizophyllum commune (裂褶菌DNA提取)
and Thompson. Nucleic Acids Res. 8:4321-4325; 1980.) 0.1 P agar For 1 L total medium: 5 ml Phosphate stock 1 ml trace elements stock 5 ml thiamine HCl 1 g L-asparagine 0.5 g MgSO4 20 g Glucose 10 g Agar 0.002 P liquid medium For 1 L total medium
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