Amplite 荧光法生物素定量检测试剂盒 | Amplit

【产品形式】：艾美捷为您提供的Amplite 荧光法生物素定量检测试剂盒The HABA Biotin Quantitation Kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution. The assay employs HABA (4?hydroxyazobenzene-2-carboxylic acid), a reagent that shows dramatic spectral changes when bound to avidin. Biotin easily displaces HABA from the HABA/Avidin complex, resulting in a decrease of absorption at 500 nm. The kit is best used to determine biotin concentration in the range from 2 to 16 礛. The assay can be performed in a cuvette or microplate format.

【背景资料】：The avidin/streptavidin-biotin interaction is the strongest known non-covalent biological interaction (Kd = 10-15 M-1) between a protein and its ligand. One avidin binds four biotins. The bond formation between biotin and avidin/streptavidin is very rapid and, once formed, is unaffected by pH, organic solvents and other denaturing agents. Both avidin and streptavidin have essentially irreversible biotin-binding properties since bound biotin can only be released by denaturing the subunits of the proteins. The tight and specific binding of biotin and its derivatives to various avidins has been extensively explored for a number of biological applications.

美国AAT Bioquest Inc.(前身是ABD Bioquest，Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域，包括显色、荧光和生物发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学，免疫学，细胞生物学和分子生物学等领域。作为AAT Bioquest Inc.的中国区域代理，艾美捷科技为中国客户提供光谱学检测技术并应用于生化、生理代谢和细胞分析领域的产品，包括显色，荧光和发光技术等全系列解决方案。

点击Amplite 荧光法生物素定量检测试剂盒查看更详细产品说明，更多应用、储存、价格、货期等信息请致电垂询AAT Bioquest中国区域授权总代理艾美捷科技有限公司。