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- 文献和实验
- 技术资料
- 英文名：
ALIZARIN RED S
- 供应商：
西宝生物
- 保存条件：
ROOM TEMPERATURE
- 库存：
25 g
- 规格：
BIOCHEMICALS
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文献和实验Alizarin Red S (Bone) and Alcian Blue (Cartilage) Staining of Cleared Skeletons
, kidneys and gut. - Alcian Blue (20mls) 2mgs alcian blue, dissolve in 0.8ml H20 add 16ml Absolute ETOH and 4mls glacial acetic acid. - Alizarin Red S Dissolve 1mg of alizarin red S in 100ml of 1% KOH. Disclaimer
Alizarin Red S (Bone) and Alcian Blue (Cartilage) Staining of Cleared Skeletons.
to alizarin red S/KOH solution, until bone is stained purple. 2-3 days. (This solution can be changed daily). Rinse in 1% KOH. ~ 3 changes several hours each. 20% Glycerol in 1% KOH 24hrs (swirl occasionally) 50% Glycerol
1. Bring sections to water 2. Acidified potassium permanganate 2 minutes 3. Wash in water 4. 1% Oxalic acid 1 minute 5. Wash in water 6. Wash in 70% alcohol (optional) 7. Stain in Weigert's Resorcin Fuchsin at room
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