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蓝冰
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200 ul (20 Western Blots)
Purified anti-STAT3参数介绍>>>>
| Clone | Poly6246 |
| Isotype | Rabbit Polyclonal |
| Reactivity | Human, Mouse, rat (does not react with STAT3b) |
| Immunogen | Peptide mapping to the carboxy terminus of mouse STAT3 p92 |
| Formulation | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin. |
| Preparation | The antibody was purified by antigen-affinity chromatography. |
| Recommended Usage | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Applications | WB-Quality tested |
| Description | STAT 3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to cytokine receptor-associated kinase activity. Phosphorylation of STAT 3 induces nuclear translocation to activate transcription. STAT3 is expressed in brain, heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. STAT 3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT 3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT 3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT 3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by Rac1 protein. STAT 3 interacts with Rac1, STAT 1, and NCOA1 proteins. The Poly6246 antibody recognizes the C-terminal region of human, mouse, and rat STAT3 and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry. The Poly6246 antibody does not react with STAT3b. |
| Storage | The antibody solution should be stored undiluted at 4°C. DO NOT FREEZE. |
品牌介绍:Biolegend公司自2002年成立以来,一直致力于为生物医药研究领域提供一流的,高质量的流式抗体、试剂盒及其它相关产品。公司聚集了一批来自PharMingen的专业杰出人士,建立了一支雄厚技术背景的专业队伍,并通过其自身的先天优势,以及与世界知名实验室和研究所的良好合作关系,以最合理的价格为广大客户提供卓越的免疫学和细胞生物学产品,并不断地开发新产品,以满足飞速发展的生命科学研究的需要。短短几年时间,Biolegend公司得到了飞速发展,已跻身全球最先进的抗体公司行列,并在2009年成为美国哈佛大家的独家抗体供应商! Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、凋亡研究及特定修饰类抗体等领域相关的试剂,用于流式、ELISA、免疫沉淀、WB、免疫荧光、免疫组化、及体内体外功能学试验。其中,Biolegend公司的流式抗体以其具有较宽范围的荧光选择及较高的性价比,为广大科研工作者所接受!同时,公司配备了一支经验丰富的技术服务队伍,为客户提供完美的配色方案及快速的售后处理服务!
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文献和实验【求助】请问用显性负性STAT3和STAT3 mRNA干扰阻断信号差异
semiboat 请问用显性负性STAT3质粒转染阻断IL-6/gp130/STAT3通道和STAT3 mRNA干扰阻断此信号通道有什么差异,在阻断此通道时是否都可以使用,园内各位XDJM有谁知道,非常感谢!! bacchante miRNA干扰比较现代化一些。 显性负作用似乎还要考虑内源蛋白的影响,如果你的启动元件活力不够高,那么你就抑制作用可能就不明显。 另外,似乎不能rescue,也就是说,似乎显性
haohuaiyong 各位战友,最近我在做磷酸化STAT3的检测,用cell signaling公司的磷酸化检测抗体与总STAT3抗体做WB,实验组(bFGF+EGF)与阳性对照的磷酸化水平是有增加的,但总STAT3在实验组是减少的而在对照组是强表达的,我看别人的文献都是将总STAT3作为阳性内参一样,应该是不变化的啊,为什么我做了好多次都是不均一的啊?实在是搞不明白,请做过这方面的战友帮忙!谢谢! dzhzh 你的灌注
maggertu 想请教个问题,做stat3的免疫组化,是选用stat3抗体,还是p-stat3抗体?看到国外大部分文章都做p-stat3,而国内有些文章用stat3抗体,搞不明白 zfyyzz00 stat3抗体只是检测总蛋白,看活性必须要做P-stat3。 在转录水平就应该做EMSA maggertu 谢谢,再请教一下,应该只有磷酸化的stat3才能进行核内转移,那么做stat3抗体
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