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- 105811
- 2025年07月14日
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25 ug
APC anti-mouse CD117 (c-kit)参数介绍>>>>
| Clone | 2B8 |
| Isotype | Rat IgG2b, κ |
| Reactivity | Mouse |
| Immunogen | Mouse bone marrow mast cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody. |
| Concentration | 0.2mg/ml |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Applications | FC - Quality tested |
| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation1 and immunohistochemistry2. The 2B8 antibody does not block c-Kit activity. |
| Application References | 1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. |
| Description | CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. |
| Storage | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
品牌介绍：Biolegend公司自2002年成立以来，一直致力于为生物医药研究领域提供一流的，高质量的流式抗体、试剂盒及其它相关产品。公司聚集了一批来自PharMingen的专业杰出人士，建立了一支雄厚技术背景的专业队伍，并通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以最合理的价格为广大客户提供卓越的免疫学和细胞生物学产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。短短几年时间，Biolegend公司得到了飞速发展，已跻身全球最先进的抗体公司行列，并在2009年成为美国哈佛大家的独家抗体供应商！ Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、凋亡研究及特定修饰类抗体等领域相关的试剂，用于流式、ELISA、免疫沉淀、WB、免疫荧光、免疫组化、及体内体外功能学试验。其中，Biolegend公司的流式抗体以其具有较宽范围的荧光选择及较高的性价比，为广大科研工作者所接受！同时，公司配备了一支经验丰富的技术服务队伍，为客户提供完美的配色方案及快速的售后处理服务！
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文献和实验C-kit Expression Identifies Cardiac Precursor Cells in Neonatal Mice
receptor c-kit. Utilizing a bacterial artificial chromosome (BAC) transgenic mouse model in which EGFP expression is placed under control of the c-kit promoter (c-kitBAC -EGFP), work from our laboratory indicates that c-kit expression identifies
, 0.1mM ßME. plus 3 U/mL Epo, 50 ng/mL Tpo, 100 ng/mL SCF, 100 ng/mL IL-6, ? ng/mL IL-3. Antibodies for Lineage Depletion: (Pharmingen) Antibodies for Sorting: (Pharmingen monoclonal rat anti-mouse)
1D 进程中发挥作用，其发挥作用的方式为创建有利于破坏胰岛β 细胞的调节环境。当前的 Panel 旨在鉴定健康人体与 T1D 患者的 NK 细胞子集在表型方面存在的差异。最终目标是构建对 T1D 疾病早期、发病前阶段具有预测意义的生物标记物。图例说明：取自健康供体的 PBMC， 并用 CD117-BV421 (c-kit)、CD7-BV510、CD57-BV605、CD8-BV650、CD14-PE、CD19-PE、CD3-PE、CD66b-PE、CD56-PE-Cy7、CD11c-APC、CD
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