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- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Universal miRNA Cloning Linker
- 供应商：
南京赛泓瑞
- 规格：
0.83 nmol
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文献和实验miRNA Expression Profiling Using Illumina Universal BeadChips
to distinguish between members of miRNA families. In addition, a universal PCR is used to amplify all targets prior to array hybridization. Using this method, highly reproducible miRNA expression profiles were generated with 100–200 ng total RNA input. The method
A T-Linker Strategy for Modification and Directional Cloning of PCR Products
The propensity of Taq polymerase to add 3′-A overhangs (1 ,2 ) to polymerase chain reaction (PCR)-amplified DNA has made possible a simple method for cloning PCR products into a T-vector (Invitrogen, San Diego, CA) (3 –
for cloning of unknown genes and analysis of expression profiling (1 -10 ). Several PCR-based strategies such as iAFLP (introduced amplified fragment length polymorphism) and TOGA (total gene-expression analysis) are currently available for global gene
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